IndraLab

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CALM1 binds WRKY53. 4 / 4
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sparser
"Second, we performed a pull-down assay, which showed that calcium affected the bonding strength of the CAM1- WRKY53 complex ( xref c)."
35887066
sparser
"To determine whether these conserved amino acids play key roles in the interaction between CAM1 and WRKY53, we mutated these amino acids to R. Only the simultaneous mutation of all four amino acids significantly affected the binding of CAM1 and WRKY53 ( xref e), which suggested that 57 V, 67 S, 68 L and 69 L form a fixed structure that combines with CAM1."
35887066
sparser
"We found that CAM1 and WRKY53 bound together when calcium was not added, while when calcium concentration increased, the protein interaction between CAM1 and WRKY53 was unbound, allowing WRKY53 to bind to the LOXs promoter and negatively regulating their expression ( xref b,c)."
35887066
sparser
"In healthy plants, CAM1 interacts with WRKY53 and WRKY53 does not bind to the LOXs promotors."
35887066