IndraLab

Statements

CS binds USP20. 4 / 4
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"As expected, the wild-type USP20 significantly decreased the polyubiquitination of PINK1, whereas the USP20-CS mutant did not (Fig.  xref A and B)."
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"HEK293 cells were transfected with a plasmid encoding the catalytically inactive USP20-CS mutant and/or treated with CCCP."
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"To confirm this, HEK293 cells were transfected for 24 h with a plasmid encoding PINK1-Myc, either alone or together with a plasmid encoding USP20-WT or its catalytically inactive USP20-CS mutant, and an immunoprecipitation assay was performed (Fig.  xref A)."
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"Immunoblot analysis of the lysates showed that the LC3-II level increased under CCCP treatment and slightly decreased with the overexpression of the USP20-CS mutant (Fig.  xref A)."
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