IndraLab

Statements

USP29 binds Flag. 10 / 10
| 10
sparser
"1) HEK293T cells transfected Flag‐USP29 and HA‐TWIST1 as indicated were lysed in NETN buffer and were incubated 4 h with Flag‐beads or HA‐beads for 4 °C."
36782089
sparser
"To define the precise region(s) in USP29 for this interaction, we constructed various Flag-USP29 mutants, and expressed a full-length HA-tagged AURKB in combination with respective Flag-USP29 fragments in 293T cells."
38233848
sparser
"To assess the potential role of USP29 in regulating AURKB ubiquitination, we co-expressed HA-AURKB, Myc-Ub, and Flag-USP29 in 293T cells, and revealed thatUSP29 markedly impaired the polyubiquitination of AURKB, which requires the DUB activity of USP29, as the catalytic-inactive C294S (substitution of cysteine 294 to serine) mutant that was reported to display a similar substrate-binding capacity [ xref ], failed to reduce AURKB polyubiquitination (Fig.  xref F)."
38233848
sparser
"IP analysis of TAK1 ubiquitination levels demonstrated that Flag-USP29 transfection significantly reduced TAK1 ubiquitination compared to the Myc-TAK1 and HA-Ub co-transfection group (Fig. xref )."
40634288
sparser
"We examined the cell activity after the H/R challenge with a CCK-8 assay and found that the cell viability of the Flag-USP29 group was significantly higher than that of control cells ( xref ), which indicates that USP29 overexpression enhanced the cells’ tolerance to the H/R challenge."
37304282
sparser
"To test this hypothesis, we ectopically expressed Flag-USP29 in gastric cancer cells and revealed that USP29 overexpression increased the AURKB protein levels (Fig.  xref A)."
38233848
sparser
"To confirm the influence of USP29 on AURKB protein stability, we transfected 293T cells with HA-AURKB and Flag-USP29, and treated the cells with CHX, a widely used inhibitor for protein translation."
38233848
sparser
"To identify the binding partners of USP29, we infected 293T cells with vector control and Flag-USP29, respectively, and Flag-bound immunoprecipitates from these cells were resolved by SDS-PAGE and visualized by silver staining (Fig.  xref A)."
38233848
sparser
"Furthermore, cotransfection experiments conducted in HEK293T cells with Myc‐ubiquitinated and HA‐TAK1 demonstrated that introduction of Flag‐USP29 significantly reduced the level of TAK1 polyubiquitination (Figure  xref )."
40040611
sparser
"The proteomic data revealed 81 and 39 putative binding proteins (with coverage > 30%) in control and Flag-USP29 immunoprecipitates, respectively (Supplementary Fig.  xref A), and AURKB, a well-established oncogenic driver, was specifically immunoprecipitated by USP29 (Fig.  xref B and Supplementary Fig.  xref B)."
38233848