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PRPF8 inhibits prp16-302. 4 / 4
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"This allowed us to analyze combinations of prp8 and U6 alleles that individually suppress prp16-302 defects to establish if they act by the same or distinct mechanisms."
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"We carried out a genetic screen for prp8 alleles within the Prp8 N-terminal domain that suppress cs defects of prp16-302."
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"Each of the prp8 alleles suppresses prp16-302 defects (Fig. 4E) to a different extent, with K603I and K611I/T exerting the strongest suppression."
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"Only a combination of T589P + K611I alleles does not follow this pattern as they act additively both for suppression of prp16-302 defects (Supplemental Fig. S1A) and for ts growth phenotypes (Supplemental Fig. S1B), consistent with their partial allele status.Together, we identified multiple alleles of cwc2, U6, and prp8 that suppress defects of prp16-302 and thus facilitate the first-to-second steps transition."
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