IndraLab

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COL9A2 binds USP13. 15 / 15
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"We noted that VAPB KO clones in the USP13-Med line displayed a remarkably altered morphology, as they became more flattened and more elongated (Sup."
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"The therapeutic potential of miR‐367 was further confirmed in vivo , in an orthotopic xenograft model of aggressive embryonal CNS tumors initiated by OCT4A‐overexpressing cells (Daoy, USP13‐MED, or USP7‐ATRT) in Balb/C nude mice."
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"Furthermore, USP13-Med and DAOY VAPB KO cells showed decreased proliferation rates compared to controls (Fig.  xref B and C)."
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"The overall survival rate of animals harboring Daoy, USP13‐MED, or USP7‐ATRT‐initiated tumors was also significantly improved by treatment with miR‐367 inhibitor (Fig. xref D)."
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"In order to assess any potential impact of doxycycline on cell proliferation, we conducted additional experiments applying doxycycline in DAOY and USP13-Med wild-type and VAPB KO cells."
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"Next, an in vivo study was conducted with a intracerebroventricular injection of ZIKV in BALB/c nude mice after period of CNS tumor establishment (1 to 2 weeks for DAOY, USP13-MED and USP7-ATRT cell lines)."
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"We noted that the number of differentially expressed genes between VAPB WT and VAPB KO cells was much higher in the USP13-Med background than in DAOY cells (Fig.  xref A)."
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"This finding agrees with the pronounced morphology change observed in USP13-Med VAPB KO s, an effect that was not detected in the DAOY VAPB KO cells."
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"In this setting, both DAOY as well as USP13-Med cell lines formed tumors in vivo and overall survival is displayed in Fig.  xref G and H. Histologic analysis of the brain of terminated mice confirmed tumors in brain tissue in every group (Fig. xref ), although VAPB KO tumors were smaller."
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"The DAOY cell line, representative of the Sonic Hedgehog (SHH) subgroup, and the USP13-Med cell line, which was obtained from a 3-year-old boy with classic medulloblastoma, subgroup 4 xref ."
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"For this, the study focused on assess three embryonal CNS tumor lineages (DAOY, USP13-MED and USP7-ATRT), as well as three non-CNS tumor cell lines from breast, colorectal and prostate cancer."
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"In USP13‐MED, miR‐367 silencing significantly inhibited proliferation of cells displaying either basal or enhanced OCT4A expression (Fig. xref B,C)."
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"72-hours after the infection, the investigators observed cell death and/or growth reduction in all the CNS tumor lineages, although infection of DAOY was less pronounced when compared to USP13-MED and USP7-ATRT."
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"Reduction of tumor growth ratio in USP7-ATRT and USP13-MED was also observed, even though DAOY cell line had a poor response to ZIKV infection, which fits in vitro findings."
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"Finally, miR‐367 silencing also significantly inhibited 3D cell invasion in tumor spheroids of both USP13‐MED and USP7‐ATRT cells (Fig. xref ), suggesting that miR‐367 silencing inhibits two key features of tumor aggressiveness, namely, stem‐like properties and invasive behavior."
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