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USP28 deubiquitinates UCK1. 3 / 3
| 3

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"We then evaluated whether USP28 could deubiquitinate UCK1."

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"In vitro deubiquitination assay further confirmed that WT-USP28, but not USP28-CA, efficiently deubiquitinated UCK1, indicating that USP28 served as direct DUB for UCK1."

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"Conversely, USP28 silencing in HEK293T markedly increased the ubiquitination of endogenous UCK1, implying that endogenous UCK1 was also a target of USP28."