IndraLab

Statements

reach
"The distal Ub–Cez2 complex structure resembled the cocrystallized Cez OTU in complex with diUb (5LRV; Figure 1)."
39782030
reach
"The persistent interaction between E173 of Cez2 and K33 of the substrate suggests that this is an important factor to stabilize the proximal Ub–Cez2 binding.Thus, for the third catalytic site residue E173, a similar dual functional role as for Cez can be suggested: it is critical for the recognition and stabilization of proximal Ub binding to Cez2 (for Cez2 Ub : 61% of the simulation time, for Cez2 Ub : 52%) and the stabilization of the Cys/His dyad by a water-mediated interaction with H367 as E173 makes a water bridge for ≥90% of the simulation time."
39782030