IndraLab

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CamB binds CALM1. 150 / 150
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"This study explores the therapeutic potential of andrographolide (AND), a bioactive compound derived from Andrographis paniculata , in mitigating cervical cancer progression using the chick embryo chorioallantoic membrane patient-derived xenograft (CAM-PDX) model."
39937205
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"Although we have shown transplanting CAM-PDXs is possible, there was no evidence for expansion of the material and given the limitations observed with passaging PDX in mice, researchers should carefully consider whether this is necessary."
40568242
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"In the CAM-PDX model, AND significantly inhibited tumor growth, primarily by reducing angiogenesis and vessel density."
39937205
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"In summary, this study successfully established a reproducible CAM-PDX model for drug evaluation and highlighted the potential of AND as a promising therapeutic candidate for cervical cancer, warranting further clinical investigation."
39937205
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"The option to test treatments in a rapid manner using the CAM-PDX platform, where turnaround time can be weeks instead of months, is highly desirable for cancers with poor prognosis such as mesothelioma, where patients may only survive a few months after their diagnosis."
40568242
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"We propose that as the model is moderate throughput, drug evaluation can be conducted on first generation (P0) CAM-PDXs, potentially further reducing numbers, as it would remove the standard practice of serial passaging in mice."
40568242
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"Chorioallantoic membrane patient-derived xenograft (CAM-PDX) models, which combine the strengths of PDX and CAM systems, offer reduced experimental costs alongside enhanced visualization and operability."
39937205
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"Studies have reported an impressive success rate of 80%–100% in CAM-PDX models across various cancer types, including glioblastoma multiforme, sarcoma, and renal cell carcinoma [ xref , xref ]."
39937205
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"In this study, we utilized a chick embryo CAM xenograft model (CAM-PDX) to evaluate the therapeutic potential of AND in cervical cancer treatment."
39937205
sparser
"This study highlights the potential of AND as a therapeutic agent and validates the CAM-PDX model as a reliable platform for further investigation into treatments for cervical cancer."
39937205
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"Furthermore, due to the success rate of generating CAM-PDX from a range of cancers ( xref ) we envisage this model accommodating most solid cancer types, bringing wider impact in the reduction and replacement of murine models in cancer research."
40568242
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"The CAM-PDX model preserves the histopathological features of SCC."
39937205
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"To further investigate, we established both SCC PDX and CAM-PDX models, observing the morphological characteristics via H&E staining."
39937205
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"Importantly, the PDX and CAM-PDX models displayed similar pathological morphologies to the patient’s tumor ( xref )."
39937205
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"Overall, the need for more accessible and physiological models of cancer, faster translation of therapies and the desire to reduce rodent model usage, makes the CAM-PDX model an attractive option."
40568242
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"The results showed that p40 and CK5/6 were consistently positive in the patient tumor, as well as in the PDX and CAM-PDX models."
39937205
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"Additionally, the CAM-PDX model versatility offers the ability to study other processes important to the development and progression of tumors (i.e. angiogenesis, invasion, metastasis, hypoxia, etc.) as well as the feasibility of employing longitudinal imaging and biosafety evaluation of promising compounds ( xref ; xref )."
36643309
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"If one successful mouse PDX is typically passaged 5-times, using 5 mice each time, then the CAM-PDX model could theoretically reduce the number of animals used per PDX by 25."
40568242
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"We expect the CAM-PDX model to be utilized for applications such testing combination therapies, formulations, drug delivery systems and novel treatment strategies."
40568242
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"We have shown that viable, vascularized mesothelioma CAM-PDXs can be generated from both fresh and frozen fragments of biopsy tissue, and that these are amenable to viability assessment using PET/CT imaging."
40568242
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"Freezing samples prior to engraftment also has the benefit of widening the availability of tissue, and therefore the appeal of the CAM-PDX model, to researchers who may be unable to readily obtain fresh tissue, for example due to a lack of local specialist hospitals or clinical collaborators."
40568242
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"The CAM-PDX model sits between PDEs and mouse PDXs, offering a middle-ground between tractability, time scale and physiological relevance."
40568242
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"Moreover, the short experimental window of the CAM-PDX model means results can be available in weeks compared to the months required to obtain meaningful data from mouse PDX models."
40568242
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"Generation of the CAM-PDX model and its histopathological characterisation."
39331331
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"Conclusion: The establishment of a CAM-PDX uveal melanoma model could elucidate the biological growth patterns and the efficacy of new therapeutic options in vivo."
36900228
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"CAM-PDXs treated with SHMT1/2 inhibitors sertraline (Fig.  xref ) or SHIN1 (Fig.  xref ) exhibited reduced tumoroid growth when compared to their respective vehicle controls."
40204966
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"The [ 68 Ga]Ga-Pentixafor uptake patterns were comparable between in vitro, in ovo and clinical data and 2-[ 18 F]FDG was avidly taken up in the patient’s liver metastasis and CAM-PDXs."
39331331
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"As a monoagent, sertraline or trimethoprim, a DHFR inhibitor (Makino et al, xref ), inhibited the growth of SETTLE cells in CAM-PDX and zebrafish-PDX, but achieved no additional benefit when used in combination (Fig.  xref )."
40204966
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"We thus propose the CAM-PDX model as an alternative in vivo model with promising translational value for CRC patients."
39331331
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"Finally, as it is common to serially passage mouse PDX ( xref ), we assessed the feasibility of this for the CAM-PDX model."
40568242
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"However, this pattern is disturbed by such models as zPDX and CAM-PDX, the strong sides of which are quick establishment, high-throughput and low-cost."
36612135
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"Combining the CAM-PDX model and FDG-PET/CT provides a quantitative in vivo platform for pre-screening of novel treatment strategies and drug combinations, with the potential for development of patient tumor avatars for predicting clinical response."
40568242
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"Our data suggests concordance between cisplatin-based chemotherapy resistance phenotypes in primary patient tumors and CAM-PDX models."
36643309
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"Tumour cells in the CAM-PDXs, the patient’s sample, and the PDOs show a highly comparable, strongly positive membrane staining."
39331331
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"Nevertheless, the non-human immune system and short observation period do not allow the solving of all research and clinical tasks only with the use of zPDX and CAM-PDX models."
36612135
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"To make this approach accessible to other laboratories as a replacement for mouse PDXs, our aim was to develop robust protocols that describe in detail how to generate CAM-PDX models of mesothelioma and monitor tumor cell composition and health to assess therapeutic responses."
40568242
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"These CAM-PDX methods can be extrapolated to other tumor types, and the tissue processing method could be applied to the generation of PDEs, or even mouse PDX models, to increase experimental efficiency thus having a wider impact across models."
40568242
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"In contrast, PDX-CAM is practically always successful regardless of glioma IDH status [ xref , xref ]."
41599159
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"To evaluate the therapeutic effects of AND, we assessed angiogenesis in the CAM-PDX model visually ( xref )."
39937205
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"In addition, CXCR4 staining was comparable in CAM-PDXs and metastatic tissue showing mainly membrane and weaker cytoplasmic staining, whereas in vitro PDOs displayed comparable levels of cytoplasmic and membrane staining."
39331331
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"We also tested select kinase inhibitor response on chemotherapy-resistant bladder cancers on the CAM-PDX using tumor growth measurements and immuno-detection of proliferation marker, Ki-67."
36643309
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"Due to the limitations of an mPDX model, alternative PDX and PDX-derived models were developed such as zebrafish patient-derived xenografts (zPDXs), chick chorioallantoic membrane patient-derived xenografts (CAM-PDXs), humanized mPDX, PDX-derived organoids (PDXO) and cell cultures (PDXC)."
36612135
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"These results suggest that the CAM-PDX model can be used to test chemotherapy doses lower than 329 μM to interrogate the effects of cisplatin and gemcitabine on MIBC engrafted tumors."
36643309
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"The presented CAM-PDX model is a physiological and translational in vivo model that complies with the 3Rs of animal experimentation [ xref ] and is of particular interest to radiopharmaceutical departments or imaging facilities without animal housing or animal ethics approval."
39331331
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"Proliferation was maintained mostly in CAM-PDX tumors to their parental counterparts ( xref E, F, G, H), although Ki-67 expression was better retained in tumors engrafted from frozen specimens compared to fresh ones ( xref M and O)."
36643309
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"Importantly, histomorphology, target protein expression, and tracer uptake patterns were conserved between the original tumour tissue and the CAM-PDXs, suggesting that organoid-derived CAM models can reflect the patient tumour phenotype and molecular characteristics."
39331331
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"Published studies report high engraftment rates (70-80%), histological concordance with tumor type and a strong correlation between CAM-PDX assay results and patient outcome ( xref , xref , xref ), supporting the CAM model as an alternative method for generating physiologically applicable PDXs that can be utilized for drug screening and personalized medicine."
40568242
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"Hence, we developed a workflow to combine CAM-PDX and PET/CT imaging to facilitate assessment of treatment response in this model."
40568242
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"The CAM-PDX assay as a true in vivo model recapitulates aspects of cancer not found in in vitro 3D models such as spheroids (e.g. vascularization), and engrafted tumors are far more accessible and amenable to manipulation than mouse PDX models."
36643309
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"However, I would caution that the immune system is not functional and that the CAM-PDX model cannot be used to evaluate the efficacy of immunotherapy approaches."
40568242
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"CAM-PDX scoring."
40568242
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"Surprisingly, not much progress has been made since then in breast cancer modeling using the CAM-PDX platform."
35697909
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"Here we describe methods to generate mesothelioma patient-derived xenografts on the CAM (CAM-PDX), and to subsequently assess these PDX nodules by preclinical imaging and histology."
40568242
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"Treatment of CAM-PDX with cisplatin and gemcitabine confirmed that the models mirrored clinical therapy resistance [ xref ]."
38630912
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"CAM-PDXs, generated from fresh or slow-frozen tissue, were well vascularized whilst maintaining the architecture and cellular composition of the patient tissue."
40568242
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"Specific objectives of the presented work were (1) the establishment of a CAM-PDX model based on PDOs derived from a liver metastasis of a CRC patient and (2) the evaluation of the presented translational pipeline (patient – in vitro PDOs – in vivo CAM-PDX) regarding morphology, histopathology, expression of CXCR4, and radiotracer uptake patterns."
39331331
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"Overall, these data show successful engraftment on the CAM following reanimation after freezing and it should be noted that despite the sparse tumor cell content of the example CAM-PDX shown in xref , it offers proof of principle of the methodology irrespective of how challenging the sample is."
40568242
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"Therefore, we aimed to (1) establish a CAM patient-derived xenograft (PDX) model based on PDOs generated from the liver metastasis of a colorectal cancer (CRC) patient and (2) to evaluate the translational pipeline (patient – in vitro PDOs – in vivo CAM-PDX) regarding morphology, histopathology, expression of C-X-C chemokine receptor type 4 (CXCR4), and radiotracer uptake patterns."
39331331
sparser
"Following in vitro re-culturing of CAM-PDXs, growth, and [ 68 Ga]Ga-Pentixafor uptake were unaltered compared to PDOs before transplantation onto the CAM."
39331331
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"Although [ 68 Ga]Ga-Pentixafor was taken up into CAM-PDXs, the uptake in the baseline and blocking group were comparable and there was only a trend towards blocking."
39331331
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"We successfully established an in vivo CAM-PDX model based on CRC PDOs."
39331331
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"As such, CAM-PDX models of bladder cancer offer unique and complimentary experiments to rodent PDX models that will save time and resources."
36643309
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"Since pleural mesothelioma is a heterogenous cancer, where tumor cell content and distribution can differ greatly between, and within patient samples ( xref ), a workflow to facilitate pre-screening of the tissue samples ( xref ) was employed to optimize selection for implanting and ensure regions with a greater proportion of tumor cells are prioritized for generating the CAM-PDXs."
40568242
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"On E14, the resulting CAM-PDXs were dissected and processed for histological assessment."
40568242
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"PDX-CAM results are available within a week, whereas PDXs in rodents take a few times longer."
41599159
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"There was also evidence on H&E of nucleated red blood cells inside cross sections of vessel-like (α-SMA positive) structures in the CAM-PDXs (white arrows, xref ), indicating perfusion of chick blood within pre-existing or new vasculature within the engrafted tissue."
40568242
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"The only discernible difference observed was that the frequency of CD4+ and CD8+ T-cells was lower in the CAM-PDXs derived from tissue frozen in the low FBS media ( xref )."
40568242
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"The histomorphological features and target protein expression of the original patient’s tissue were mirrored in the in vitro PDOs, and particularly in the in vivo CAM-PDXs."
39331331
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"Liver metastasis organoids derived from the CRC patient, inoculated as pre-formed organoids, were successfully grown on the CAM, generating large, viable, and vascularised CAM-PDXs."
39331331
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"Chick Chorioallantoic Membrane Patient-Derived Xenografts (CAM-PDXs)."
36612135
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"Histopathological characterisation of CAM-PDXs in comparison with the patient’s liver metastasis and the in vitro PDOs is shown in Fig.  xref ."
39331331
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"Briefly, CAM-PDX generated from frozen epithelioid fragments were dissected on E14 (P0), cut in half and transplanted to new E7 eggs (P1)."
40568242
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"CAM-PDXs are characterized by high successful transplantation rate which reaches to 80–100% for glioblastoma, sarcoma, nasopharyngeal carcinoma, and renal cell carcinoma [ xref ]."
36612135
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"The advantages of CAM-PDX model are low-cost, easily-visualized and manipulatable ( xref )."
36612135
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"Establishing of CAM-PDXs and obtaining results take about 2 weeks."
36612135
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"Therefore, PDX models are extensively exploited in clinical trials for the treatment of patients with metastatic renal cell carcinoma (CAM-PDX model, NCT04602702), recurrent metastatic head and neck squamous cell carcinoma (mPDX model, NCT02752932), metastatic prostate cancer (mPDX model, NCT03786848)."
36612135
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"Tumour cells within the CAM-PDXs showed strong staining for PAS, especially on their luminal side, indicating mucus production, similar to the metastatic tissue, while in vitro PDOs were largely PAS-negative."
39331331
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"For example, the first step of drug screening should be carried out using high-throughput models such as cell cultures, spheroids, organoids, as well as PDX-derived cell cultures and organoids, the second step of screening should be based on high-throughput animal models such as zPDX and CAM-PDX, and finally, activity of the most promising candidates should be confirmed using an mPDX model."
36612135
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"Macroscopic and microscopic examination of CAM-PDXs revealed a high level of vascularisation as visualised by Desmin staining."
39331331
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"CAM-PDX re-culturing and in vitro [ 68 Ga]Ga-Pentixafor uptake in PDOs."
39331331
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"Re-culturing CAM-PDXs resulted in viable post-ovo PDO lines ( n  = 3, Fig.  xref B) exhibiting morphology and growth characteristics similar to the original PDO line (Fig.  xref A). [ 68 Ga]Pentixafor was moderately taken up in both pre-ovo and post-ovo PDOs (Fig.  xref C, Supplementary Table 1)."
39331331
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"[ 68 Ga]Ga-Pentixafor and 2-[ 18 F]FDG uptake into CAM-PDXs were investigated by ex vivo gamma counting."
39331331
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"Despite the notable accumulation of [ 68 Ga]Ga-Pentixafor in CAM-PDXs, there was only a trend towards blocking, but no significant difference compared to the blocking group (11.23 ± 2.7 vs. 8.91 ± 2.1%ID/g, p  = 0.3047, unpaired t -test, n  = 3)."
39331331
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"This longitudinal imaging suggests a weak specific uptake of [ 68 Ga]Ga-Pentixafor into CAM-PDXs (6.64 and 5.25%TA/cc for baseline and blocking, respectively, n  = 1)."
39331331
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"To visualize viable mesothelioma cells within the CAM-PDXs in ovo , we required an imaging modality that is suitable for unlabeled tissue and we therefore utilized [ 18 F]-FDG PET/CT. [ 18 F]-FDG is a non-metabolizable glucose analogue widely used as a radiotracer in oncology, including mesothelioma ( xref ), as elevated glucose uptake is commonly observed in cancer cells due to the Warburg effect ( xref )."
40568242
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"This work reports the successful establishment of a CAM-PDX model derived from liver metastasis organoids from a CRC patient highly recapitulating the patient’s characteristics: The morphology, histopathology, and CXCR4 expression of the patient’s liver metastasis were reflected in in vitro PDOs and especially in CAM xenografts underlining the translational value of this alternative in vivo model for CRC patients."
39331331
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"PET imaging after intravenous administration of [ 18 F]-FDG revealed tracer uptake ( xref ) in the CAM-PDX which were established from the same cryopreserved epithelioid tissue shown in xref - xref . [ 18 F]-FDG uptake indicates that the CAM-PDXs were sufficiently well vascularized to accumulate administered substances, such as the radiotracer, from circulating blood, and that they are metabolically active."
40568242
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"Using IHC, we demonstrated extensive vascularisation of the CAM-PDXs which was further confirmed by radiotracer uptake into the grafts."
39331331
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"Quantification of the PET signal, reported as total accumulated SUV across the entire CAM-PDX (SUVacc), showed similar uptake in CAM-PDXs (Two Sample t-test, p = 0.76), irrespective of the cryopreservation media used ( xref ), suggesting that both freezing methods preserve viable tissue that can be reanimated for engraftment."
40568242
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"CC3 staining, performed to assess the extent of apoptosis in the CAM-PDX model, revealed a low number of CC3-positive cells localised mainly in the lumens of the glands, further confirming the high viability of the grafts."
39331331
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"The CAM-PDXs consist not only of tumour cells and blood vessels but also of CAM-connective tissue, as indicated by histological analyses."
39331331
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"[ 68 Ga]Ga-Pentixafor PET/MRI was performed under baseline and blocking conditions in the same egg on two subsequent days suggesting a weak specific uptake into CAM-PDXs."
39331331
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"CAM-PDX leverages an easily accessible engraftment scaffold and vascular-rich, immunosuppressed environment for the engraftment of PDX tumors and subsequent functional studies."
36643309
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"We optimized engraftment conditions for primary MIBC tumors using the CAM-PDX model and tested concordance between cisplatin-based chemotherapy response of patients to matching PDX tumors using tumor growth coupled with immunohistochemistry markers of proliferation and apoptosis."
36643309
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"Albeit, ex vivo assessment of [ 68 Ga]Ga-Pentixafor uptake in CAM-PDXs did not reveal a significant difference between the baseline and blocking group (mean uptake 11.23 vs. 8.91%ID/g), possibly due to the low CXCR4 expression combined with a small amount of inoculated cells (1 × 10 5 PDOs) and a natural egg-to-egg variation."
39331331
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"Further, proteogenomic informed kinase inhibitor use on MIBC CAM-PDX models suggests a benefit from integration of rapid in vivo testing of novel therapeutics to inform more complex, pre-clinical mouse PDX experiments for more effective clinical trial design aimed at achieving optimal precision medicine for patients with limited treatment options."
36643309
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"To confirm the fidelity of PDX tumors on the CAM, we compared the histological properties of original parent tumors to CAM-PDXs engrafted as tumor fragments."
36643309
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"To determine viability of tumors engrafted on CAM-PDX we assessed proliferation and apoptosis using Ki-67 and cleaved caspase 3 markers, respectively."
36643309
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"Results from these dose response curve studies suggest that EGFR/HER2, CDK4/6, and FGFR pathways are not critical for the viability of chick embryos at the doses and times given ( xref ) and can therefore be safely used on the CAM-PDX model without compromising embryos."
36643309
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"Clinical resistance to cisplatin-based chemotherapy of pre-NAC MIBC tumors is maintained in CAM-PDX."
36643309
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"Furthermore, treatment with chemotherapy increased Ki-67 and cleaved caspase 3 compared to vehicle treatment on CAM-PDX ( xref C)."
36643309
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"We describe herein an in vivo method to engraft primary tumors from MIBC patients on the chicken egg CAM-PDX model to rapidly generate models suitable for pre-clinical therapeutic testing."
36643309
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"After establishing a pre-NAC resistant model of MIBC on the CAM, we sought to demonstrate the feasibility of the CAM-PDX model by performing proof-of-concept studies using the selected kinase inhibitors tested in xref ."
36643309
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"The CAM-PDX is continuously growing as an advanced 3D, in ovo model to leverage fertilized chicken embryo eggs for the engraftment of patient-derived tumor specimens."
36643309
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"In addition, the CAM-PDX model is able to preserve the histological architecture, cell proliferation, and apoptotic phenotypes of matched parental tumors."
36643309
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"This discrepancy may be due to the small number of bladder cancer CAM-PDXs we have compared in our study and will likely improve as we increase our sample size."
36643309
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"Furthermore, CAM-PDX model experiments demonstrate concordance with clinical outcomes of tumors in patients that received cisplatin-based chemotherapy and proof of principle studies support the feasibility of using this platform as a way to test small molecule inhibitors."
36643309
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"Results from the current study demonstrate that the CAM-PDX model provides a niche for mimicking growth characteristics of the parental human tumors."
36643309
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"Due to the immunodeficient nature of the chick embryo and the abundant vascularization of the CAM, this in vivo model has proven to be very useful for the implantation of various types of cancer cell lines and patient-derived tumor cells or explants. xref , xref The CAM-PDX model is well suited for rapid tumor engraftment and subsequent testing of therapeutic approaches."
36821432
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"Establishment of a High-efficiency Glioma CAM-PDX Model that Retains the Histopathological and Molecular Characteristics of the Original Tumor."
36821432
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"To develop the glioma CAM-PDX model, we used an ex-ovo chicken embryo culture system to allow broad access to the CAM for external manipulations, including tumor implantation and drug treatments."
36821432
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"Dose determination for CDDP and AND in CAM-PDX assay."
39937205
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"To determine the appropriate doses of CDDP and AND for the CAM-PDX assay, we treated 50 non-transplanted, 11-day-old chicken embryos, dividing them into five groups with varying doses of CDDP."
39937205
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"These doses enable effective evaluation of the individual and combined effects of CDDP and AND in CAM-PDX models."
39937205
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"These results suggest that the CAM-PDX model is appropriate for assessing sensitivity/resistance to chemotherapeutic drugs commonly used to treat HGGs at doses corresponding to the ones administered to patients."
36821432
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"We next evaluated the concordance between tumorigenicity in the CAM-PDX model and patient clinical outcome."
36821432
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"Kaplan-Meier analysis of PFS curves indicates that recurrence tends to occur later in patients with low tumor fragment uptake on CAM compared to those with a high uptake ( xref ), whereas a significant association was observed between CAM-PDX engraftment and overall patient survival ( xref )."
36821432
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"These results indicate that higher CAM-PDX tumorigenicity is associated with poorer prognosis in glioma patients."
36821432
sparser
"To determine whether the CAM-PDX model can be used as an Avatar model to predict clinical responses in patients with glioma, we retrospectively evaluated treatment response in 15 HGG patients and their corresponding CAM-PDX models that were treated with optimized doses of TMZ, carboplatin, or vehicle alone."
36821432
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"Treatments were considered effective in CAM when they resulted in a >30% and significant inhibition of tumor volume, according to the internationally standardized clinical Response Evaluation Criteria In Solid Tumors. xref These criteria were adapted to the CAM-PDX model by using the control group as a reference."
36821432
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"Patient response was classified as either CR, PR, or SD, which are generally considered therapeutically beneficial in a clinical setting, or PD. xref summarizes the chemosensitivity profiles of these 14 glioma patients who generated 15 matched CAM-PDX models, 2 of which were derived from the same patient."
36821432
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"In contrast, stabilization of the patient’s pathology was observed in cases where the drug significantly inhibited tumor growth in CAM-PDX ( xref , xref )."
36821432
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"AND inhibits angiogenesis in the CAM-PDX model of cervical cancer."
39937205
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"For the first CAM-PDX model, which was established after initial tumor resection, drug treatment of xenografted tumors showed significative tumor volume inhibition with carboplatin, whereas TMZ had no significant effect ( xref )."
36821432
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"In the second CAM-PDX model generated following this second surgery, carboplatin had no effect on xenograft volume ( xref ), which again was associated with a lack of clinical response of the patient to this drug ( xref )."
36821432
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"Taken together, these results further support the idea that drug responses in CAM-PDX closely mimic patient clinical outcomes."
36821432
sparser
"For this study, fresh tissue fragments from 60 glioma patients were implanted onto CAMs and 59 CAM-PDX models were successfully established."
36821432
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"This engraftment rate in the CAM-PDX model was higher than those observed in heterotopic mouse PDX glioma models, which ranged from 38% to 69%, xref , xref but more comparable to, yet higher than, the orthotopic model of brain tumors, which showed a 76%–90% success rate. xref , xref Of note, all 25 IDH-mutated samples successfully engrafted in the CAM model."
36821432
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"The CAM-PDX model, which allows the engraftment of tumors from patients with different types of gliomas regardless of their IDH status, would therefore be well suited for the evaluation of new therapeutic strategies designed to target the different genetic types of gliomas."
36821432
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"For instance, features of the original tissue such as pleomorphic astrocytic cells or cells expressing stem-like and glial markers also populated the matching CAM-PDX."
36821432
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"In fact, this sample was identified as a giant cell glioblastoma, a rare variant of glioblastoma IDH -wild type characterized by frequent TP53 mutations, a marker associated with GBM subclonal heterogeneity. xref , xref These results highlight that, while CAM xenografts generally retain key features of the parental tumor, further studies will be needed to define whether this GBM variant should be included in an eventual glioma CAM-PDX precision medicine platform."
36821432
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"CAM-PDXs have been shown to maintain the heterogeneity, pathophysiology, and major morphological and cellular features of the original tumors [ xref , xref ]."
36612135
sparser
"The glioma CAM-PDX model established herein can overcome some of these limitations."
36821432
sparser
"Even though further evaluation of the correlation between patient responses and corresponding PDX in a larger prospective setting will be required, these data suggest that the glioma CAM-PDX model has a high potential to become a valuable platform for modeling clinical response in patients."
36821432
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"Therefore, we used H&E staining under light microscopy to evaluate tumor morphology in CAM-PDX models ( xref )."
39937205
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"Tumors transplanted into the CAM-PDX model were poorly differentiated, with tumor cells displaying irregular, large, round, or oval shapes."
39937205
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"The CAM-PDX model may also be used with other types of cancer."
40568242
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"One promising advancement is the CAM-PDX model, which utilizes fertilized chicken eggs to implant patient-derived tumor specimens [ xref ]."
39937205
sparser
"Moreover, the CAM-PDX models preserved the original tumor tissue structure, protein expression profile, cell proliferation characteristics, and apoptotic phenotype of the parental tumors."
39937205
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"Notably, the feasibility of the CAM-PDX model as a testing platform for cervical cancer therapies was validated by demonstrating its concordance with clinical outcomes in patients receiving CDDP-based chemotherapy."
39937205
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"This underscores the potential of CAM-PDX models in the development and validation of new treatment strategies for cervical cancer."
39937205
sparser
"Our results demonstrate that the CAM-PDX model provides a suitable tumor microenvironment that accurately simulates the growth characteristics of human tumors."
39937205
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"Establishing a cervical cancer model using the CAM-PDX platform represents a highly valuable strategy, providing new opportunities for investigating mechanisms of drug action [ xref ]."
39937205
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"The article describes the processing of patient tissue, validation of concordance between the original tissue and the passaged tissue, implantation of the tissue sample, and imaging of the CAM-PDX using PET/CT."
40568242
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"Overall, our results underscore the utility of the CAM-PDX models for evaluating therapeutic strategies and highlight the potential of AND in combination with CDDP for treating cervical SCC."
39937205
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"The primary rationale behind developing the CAM-PDX method is the potential to reduce animal usage in cancer studies and expedite the personalized medicine approach to drug screening, without losing the translational capability of similar models."
40568242
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"Therefore, CAM-PDX models have gained increased importance as a valuable platform in precision medicine ( xref )."
35642667
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"But in a broader sense it demonstrates the use of the CAM-PDX system in studying cancers while maintaining similarity to the original patient tissue."
40568242
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"I agree with the authors conclusions that the CAM-PDX system is a viable alternative to cell or animal models in studying some aspects of cancer."
40568242
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"The manuscript entitled " Methodology for generating chorioallantoic membrane patient-derived xenograft (CAM-PDX) models of pleural mesothelioma and performing preclinical imaging for the translation of cancer studies and drug screening" written by Schulze and and his colleagues is a study that describes the procedure to generated a CAM-PDX from mesothelioma."
40568242