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"Moreover, immunofluorescence staining confirmed the interaction of USP2 and EBF2 in primary brown adipocytes (Figure 6K)."

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"To further determine the interaction between USP2 and EBF2, we constructed Flag-USP2 and HA-EBF2 plasmids and transfected them individually or together into HEK293T cells."

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"The association between Flag-USP2 and HA-EBF2 was detected in these cells (Figure 6I,J)."