IndraLab

Statements

JOSD2 binds KRAS. 19 / 19
| 8 11
sparser
"As shown in Fig.  xref , both wild-type JOSD2 and its catalytic mutants interact with KRAS, suggesting that the catalytic activity of JOSD2 is not necessary for KRAS binding to JOSD2."
40240366
sparser
"In addition, we also observed an interaction between exogenous JOSD2 and endogenous KRAS as well as exogenous KRAS and endogenous JOSD2 (Fig.  xref )."
40240366
sparser
"Moreover, the protein-protein interaction between endogenous JOSD2 and endogenous KRAS in SW620 and HCT-116 cells was further demonstrated in our co-immunoprecipitation assays (Fig.  xref )."
40240366
sparser
"To gain further insight into how JOSD2 regulated KRAS turnover and activity through its DUB activity, we first examined the exogenous interaction between JOSD2 and KRAS."
40240366
reach
"Moreover, the protein-protein interaction between endogenous JOSD2 and endogenous KRAS in SW620 and HCT-116 cells was further demonstrated in our co-immunoprecipitation assays (Fig. 2e)."
40240366
reach
"To gain further insight into how JOSD2 regulated KRAS turnover and activity through its DUB activity, we first examined the exogenous interaction between JOSD2 and KRAS."
40240366
sparser
"Considering JOSD2 regulated KRAS in an enzyme activity-dependent manner, we next asked whether JOSD2 binding to KRAS relying on its catalytic activity."
40240366
reach
"Considering JOSD2 regulated KRAS in an enzyme activity-dependent manner, we next asked whether JOSD2 binding to KRAS relying on its catalytic activity."
40240366
reach
"As shown in Fig. 2b, both wild-type JOSD2 and its catalytic mutants interact with KRAS, suggesting that the catalytic activity of JOSD2 is not necessary for KRAS binding to JOSD2.In addition, we also observed an interaction between exogenous JOSD2 and endogenous KRAS as well as exogenous KRAS and endogenous JOSD2 (Fig. 2c, d)."
40240366
reach
"To further demonstrate that the interaction between KRAS and JOSD2 is influenced by physiological or pathological conditions, we then introduced EGF and found that EGF stimulation indeed significantly promoted the MAPK signaling pathway (Supplementary Fig. 2f), which is consistent with the previous study ."
40240366
reach
"Moreover, we found that the interaction between JOSD2 and KRAS was greatly increased by EGF, suggesting that this interaction might be influenced by KRAS activity stimulators."
40240366
reach
"In our study, we demonstrated the interaction between JOSD2 and KRAS by exogenous/endogenous immunoprecipitation assays."
40240366
reach
"These data collectively demonstrated that JOSD2 directly binds to and deubiquitinates KRAS."
40240366
sparser
"JOSD2 interacts with KRAS."
40240366
sparser
"To further demonstrate that the interaction between KRAS and JOSD2 is influenced by physiological or pathological conditions, we then introduced EGF and found that EGF stimulation indeed significantly promoted the MAPK signaling pathway (Supplementary Fig.  xref ), which is consistent with the previous study xref ."
40240366
sparser
"Moreover, we found that the interaction between JOSD2 and KRAS was greatly increased by EGF, suggesting that this interaction might be influenced by KRAS activity stimulators. (Supplementary Fig.  xref )."
40240366
sparser
"Aforementioned data suggested that JOSD2 interacts with KRAS and positively regulates KRAS turnover and activity in an enzymatic activity-dependent manner."
40240366
sparser
"In our study, we demonstrated the interaction between JOSD2 and KRAS by exogenous/endogenous immunoprecipitation assays."
40240366
sparser
"These data collectively demonstrated that JOSD2 directly binds to and deubiquitinates KRAS."
40240366