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"Next we purified ubiquitin conjugates from L18-MDP-stimulated Mysm1 − /− BMDMs by TUBE and incubated them with recombinant MYSM1 (Rec-MYSM1) and compared the ubiquitin restriction pattern of Ub-RIP2 with that of AMSH, UCHL3, OTULIN or a combination of these three DUBs (Fig.  xref )."