IndraLab

Statements

USP37 binds PCNA. 19 / 19
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"Various molecular assays, including colony formation, immunofluorescence, immunoprecipitation, and DNA replication restart, were employed to examine the physical interaction between USP37 and PCNA, as well as its physiological effects in osteosarcoma cells."
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"Additionally, molecular docking studies were conducted to gain insight into the nature of the interaction between USP37 and PCNA."
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"The interaction between USP37 and PCNA is involved in the regulation of replication stress, and disrupting it could potentially trigger synthetic lethality in osteosarcoma."
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"We observed increased stabilization of PCNA in cells overexpressing USP37 (Fig. 6B) compared to cells without USP37 overexpression (Fig. 6A), indicating a potential interaction between USP37 and PCNA To test this hypothesis, we treated cells with two cytotoxic agents, HU and cisplatin, and performed immunoprecipitation using anti-USP37 antibody, which was subsequently probed with anti-PCNA antibody."
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"Validation of USP37 interaction with PCNA using molecular docking."
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"This interaction between USP37 and PCNA plays a vital role in stabilizing different replication factors at the replication fork."
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"Our findings show that in osteosarcoma cells, USP37's interaction with PCNA plays a role in supporting the stability of the replication fork."
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"This interaction between USP37 and PCNA plays a vital role in stabilizing different replication factors at the replication fork."
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No evidence text available
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"Our study has found that USP37 interacts with PCNA, a central replication factor, and has a higher affinity for PCNA-DNA complex than for PCNA alone."
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No evidence text available
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"We observed increased stabilization of PCNA in cells overexpressing USP37 (Fig.  xref B) compared to cells without USP37 overexpression (Fig.  xref A), indicating a potential interaction between USP37 and PCNA To test this hypothesis, we treated cells with two cytotoxic agents, HU and cisplatin, and performed immunoprecipitation using anti-USP37 antibody, which was subsequently probed with anti-PCNA antibody."
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"Our findings show that in osteosarcoma cells, USP37's interaction with PCNA plays a role in supporting the stability of the replication fork."
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"We observed that USP37 interacted with PCNA, and this interaction increased upon treatment with HU and cisplatin (Fig.  xref C)."
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"Our docking studies demonstrated that the USP37 PH domain interacts stably with the PCNA-DNA complex, forming five interactions (Fig.  xref B)."
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"Various molecular assays, including colony formation, immunofluorescence, immunoprecipitation, and DNA replication restart, were employed to examine the physical interaction between USP37 and PCNA, as well as its physiological effects in osteosarcoma cells."
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"Endogenous USP37 also interacted with PCNA in both the absence and presence of replication stress, and this interaction was confirmed through both forward and reverse immunoprecipitation (Fig.  xref C, D)."
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sparser
"Additionally, molecular docking studies were conducted to gain insight into the nature of the interaction between USP37 and PCNA."
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sparser
"The interaction between USP37 and PCNA is involved in the regulation of replication stress, and disrupting it could potentially trigger synthetic lethality in osteosarcoma."
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