IndraLab

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"These constructs, along with full-length SFB-USP39, were co-transfected into HEK293T cells, and SFB-USP39 was pulled down using S-protein agarose beads. xref B shows that both full-length ETS2 and three ETS2 mutants, but not 171–362, interacted with USP39."

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"Then, we examined the interaction of SFB-USP39 with these immunoprecipitated proteins."

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"To investigate this, we co-transfected MYC-ETS2 with SFB-USP39 or SFB-GFP plasmids into HEK293T cells and separated the cytoplasmic and nuclear fractions of the cell lysates."

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"To do so, we co-transfected the ETS2-responsive reporter plasmid with MYC-ETS2 alone, SFB-USP39 alone, or both plasmids into HEK293T cells."