IndraLab

Statements

ZRANB1 binds HECTD1. 24 / 24
9 | 3 12
reach
"Although we attempted to validate the interaction between endogenous TRABID and endogenous HECTD1, none of the four TRABID antibodies that we tried could detect endogenous TRABID (Data not shown)."
| PMC
biogrid
No evidence text available
23277359
reach
"First, we validated the interaction between TRABID and HECTD1 using GST-tagged TRABID NZF 1–3, in line with our previous work (Fig. 6C)."
35915203
biogrid
No evidence text available
33853758
biogrid
No evidence text available
33853758
biogrid
No evidence text available
33853758
sparser
"Thus, this study identifies HECTD1 as a mammalian E3 ligase that assembles branched K29/K48 chains and also establishes TRABID-HECTD1 as a DUB/E3 pair regulating K29 linkages."
| PMC
sparser
"Therefore, we further explored the TRABID-HECTD1 interaction in order to reconcile and also expand on these observations."
| PMC
sparser
"Although we attempted to validate the interaction between endogenous TRABID and endogenous HECTD1, none of the four TRABID antibodies that we tried could detect endogenous TRABID (Data not shown)."
| PMC
biogrid
No evidence text available
23277359
sparser
"Therefore, we further explored the molecular basis for the TRABID-HECTD1 interaction and hypothesized that HECTD1 could assemble ubiquitin chains that are subject to TRABID DUB activity."
| PMC
biogrid
No evidence text available
23277359
biogrid
No evidence text available
33711283
sparser
"For example, TRABID interacts with the E3 ligase HECTD1 and hydrolyzes K63-linked polyubiquitination of HECTD1 [ 12 , 13 ]."
35944360
sparser
"Thus, this study identifies HECTD1 as a mammalian E3 ligase that assembles branched K29/K48 chains and also establishes TRABID-HECTD1 as a DUB/E3 pair regulating K29 linkages."
33853758
biogrid
No evidence text available
35944360
biogrid
No evidence text available
19615732
sparser
"Together, our data identify TRABID-HECTD1 as the first DUB-E3 pair regulating K29-linked polyubiquitin chains."
| PMC
sparser
"Transient and genetic loss-of-function/rescue assays in mammalian cells showed that TRABID-HECTD1 is a functional DUB-E3 pair, with TRABID required to maintain HECTD1 stability."
| PMC
sparser
"First, we validated the interaction between TRABID and HECTD1 using GST-tagged TRABID NZF 1–3, in line with our previous work (Fig.  xref C)."
35915203
sparser
"In line with our previous data, the TRABID-HECTD1 interaction was dependent on the ubiquitin binding property of TRABID NZF 1–3, as TY > LV mutations in each of these UBDs abrogated binding to endogenous HECTD1 in pull-down assays (Fig.  xref D, lane 3 vs 2) xref , xref ."
35915203
reach
"GST pull-down was also used to further validate the interaction between TRABID and HECTD1."
| PMC
sparser
"This revealed that the TRABID-HECTD1 interaction, as well as the polyubiquitylated species recognized by TRABID NZF 1–3, can indeed be captured in all stages of the cell cycle, including during mitosis."
35915203
sparser
"Thus, this study identifies HECTD1 as a mammalian E3 ligase which assembles branched K29/K48 chains and also establishes TRABID-HECTD1 as a DUB/E3 pair regulating K29 linkages."
33380427