"We determined that EGF activates phosphorylation of Akt, mTOR, p70S6 kinase, ribosomal protein S6, eIF4E and 4E-BP1 in a PI-3-kinase dependent manner, and PI-3-kinase activation may prevent lactogenic differentiation in HC11 mammary epithelial cells by regulating the synthesis of proteins."
"Indeed, western blotting analysis suggests that ouabain treatment reduced EGF-induced phosphorylation of ERK 1/2 and P70S6K (the downstream target of mTOR pathway), but not AKT or 4EBP1 ( xref )."
"In contrast, in MCF10A cells, and those expressing SCRIBWT, EGF stimulation did not significantly increase p70S6K phosphorylation up to 45 minutes after stimulation."
"EGF stimulation induced phosphorylation of p70 in HepG2 and Hep3B, and to a lesser extent in Huh7 cells."
"Also harmine treatment was able to interfere with EGF-stimulated S6K1 phosphorylation, although to a lesser degree ( Figure S3 B)."
"BI-D1870 and SL0101 showed no important change in EGF-induced phosphorylation of rpS6 and p70S6K ( Fig. 7 F and G)."
"BI-D1870 showed no important change in EGF-induced phosphorylation of rpS6 and p70S6K ( Fig. 7 F and G)."
"Mechanistically, MEL specifically inhibited the EGF induced HIF-1alpha expression by suppressing the phosphorylation of ERK, mTOR and p70S6K."
"EGF induced a rapid phosphorylation of Akt, tuberin, p70S6K and 4E-BP1 ( xref )."
"As shown in xref , EGF-induced phosphorylation of mTOR, 4E-BP1, and P70S6K was significantly decreased in SiNUAK1/H1299 cells compared with Scr/H1299 cells."
"Ouabain inhibited EGF-induced phosphorylation of Rac/cdc42, profillin, ERK1/2 and P70S6K. The NKP may offer a novel therapeutic target in breast cancer patients who have developed metastasis, aiming to improve therapeutic outcomes and enhance survival rate."
"We showed that EGF treatment induced the internalization of NKP from the cell membrane of pII cells to the cytoplasmic compartment and ouabain treatment inhibited EGF induced phosphorylation of p70S6K and ERK 1/2 in agreement with previous reports [ xref , xref ]."
"No interference between the MEK/ERK pathway and 4E-BP1 phosphorylation was detected, whereas p70S6K phosphorylation induced by EGF was under a U0126-sensitive regulation."
"Everolimus efficiently inhibited EGF dependent phosphorylation of p70S6K in all the cell lines, without affecting or even inducing phosphorylation of Akt and MAPK."
"However, LY294002 effectively inhibited the EGF-induced phosphorylation of AKT and p70 S6K1, as AKT and p70 S6K1 are downstream effectors of PI3K [29,30] ."
"EGF, FGF2, and PDGF-BB stimulation activated mTOR signaling using p70S6K1 phosphorylation as the readout (XREF_FIG)."
"EGF also stimulates the phosphorylation of P70S6 kinase (P70S6K), the downstream target of mTORC1."
"No interference between the MEK and ERK pathway and 4E-BP1 phosphorylation was detected, whereas p70S6K phosphorylation induced by EGF was under a U0126 sensitive regulation."
"Ouabain inhibited EGF-induced phosphorylation of Rac/cdc42, profillin, ERK1/2 and P70S6K."
"Consistent with this, epidermal growth factor-induced Thr(389) phosphorylation in wild type S6K1 persisted for up to 120 min, whereas kinase-inactive mutants of S6K1 displayed only a reduced and transient increase in Thr(389) phosphorylation."
"MEL specifically inhibited the EGF induced HIF-1alpha expression by suppressing the phosphorylation of ERK, mTOR and p70S6K and also blocked the EGF induced DNA binding activity of HIF-1alpha and the secretion of VEGF."
"EGF induced a rapid phosphorylation of Akt, tuberin, p70S6K and 4E-BP1 (XREF_FIG)."
"EGF induced activation of Akt results in mTOR dependent p70S6 kinase phosphorylation and inhibition of HC11 cell lactogenic differentiation."
"In this study, EGF significantly stimulated HTR8 and SVneo cell migration and the phosphorylation of AKT, ERK1/2 and p70S6K in a concentration dependent manner."