IndraLab

Statements

SCN11A binds PBS. 6 / 6
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"The labelled heparin, in phosphate buffer saline (PBS), was kept at C4±C. Heparin binding assay Polyurethane disks were conditioned with 1 ml of PBS-NaN3 (0.14 M sodium chloride, 0.02% sodium azide, 0.01 M phosphate, pH 6.8) for 12 h at 22±C. Then, 125I-Hep (5 £ 104 cpm) was added to Eppendorf tubes, each of them containing a single polyurethane disk immersed in 0.5 ml of PBS-NaN3, and incubated in an end-over-end mixer for increasing times (from 0.5 to 24 h)."
10903035
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"The cells were washed three times in 200 μl of FACS buffer and then once in 200 μl of PBS-NaN 3 ."
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"Finally, cells were washed and resuspended in PBS-NaN 3 ."
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"MNC were isolated by density gradient ( 1.077 g/ml) using Ficoll-Lymphoprep and suspended in 80-100 p1 of phosphate buffered saline (PBS) containing 0.1% sodium azide (PBS-NaN?)."
10609784
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"After two washings with PBS-NaN3, the associated radioactivity was counted with the ° -counter."
10903035
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"Chitosan scaffolds loaded with the plasmid DNA were incubated with PBS-NaN 3 for up to 7 days at 37 °C. The DNA released into the supernatant was analyzed after 1, 2 and 7 days of exposure of the scaffolds and electrophoresed on a 1% agarose gel and imaged."
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