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Statements

HMGB1 binds USP13. 19 / 19
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"In this study, we demonstrated, based on LC–MS/MS, immunoprecipitation, and PLA studies, that the deubiquitinase USP13 interacts with HMGB1."
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"Further study including the binding of USP13 to HMGB1 by proinflammatory stimulation is under investigation."
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"To confirm the interaction between HMGB1 and USP13, PLA was performed using the Duolink in situ fluorescence kit (Sigma-Aldrich)."
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"In this study, we investigated the effect of isopeptidase T-3, also called USP13, on HMGB1, since HMGB1 ubiquitination has rarely been reported.To confirm whether USP13 binds to HMGB1, both Myc-HMGB1 and Flag-tagged USP13 (Flag-USP13) or USP10 and USP15 plasmids were overexpressed in HEK293T cells for co-immunoprecipitation."
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"As shown in Fig. 1B and 1C, HMGB1 binds to USP13 but not to either USP10 or USP15 after immunoprecipitation with Myc."
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"HEK293T cells were immunoprecipitated with an anti-USP13 antibody for endogenous binding, and the binding of endogenous HMGB1 to USP13 is shown (Fig. 1D)."
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"As expected, Spautin-1 treatment decreased the binding of HMGB1 to USP13, suggesting that inhibition of the deubiquitinase activity of USP13 affects its binding to HMGB1 (Fig. 1F)."
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"The interaction between HMGB1 and USP13 was also observed using a proximity ligation assay (PLA) in HEK293T cells overexpressing HMGB1 and USP13 (Fig. 1G)."
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"Further study including the binding of USP13 to HMGB1 by proinflammatory stimulation is under investigation."
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"USP13 Interacts with HMGB1."
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"To confirm whether USP13 binds to HMGB1, both Myc-HMGB1 and Flag-tagged USP13 (Flag-USP13) or USP10 and USP15 plasmids were overexpressed in HEK293T cells for co-immunoprecipitation."
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"As shown in Fig.  xref B and 1C, HMGB1 binds to USP13 but not to either USP10 or USP15 after immunoprecipitation with Myc."
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"HEK293T cells were immunoprecipitated with an anti-USP13 antibody for endogenous binding, and the binding of endogenous HMGB1 to USP13 is shown (Fig.  xref D)."
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"As expected, Spautin-1 treatment decreased the binding of HMGB1 to USP13, suggesting that inhibition of the deubiquitinase activity of USP13 affects its binding to HMGB1 (Fig.  xref F)."
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"The interaction between HMGB1 and USP13 was also observed using a proximity ligation assay (PLA) in HEK293T cells overexpressing HMGB1 and USP13 (Fig.  xref G)."
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