IndraLab

Statements

VSD activates KCNQ1. 3 / 3
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"Modification of I230C is voltage dependent in the presence of endogenous PIP 2 and after PIP 2 depletion by CiVSP, confirming that PIP 2 is not required for VSD activation in KCNQ1."
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"To test if PIP 2 affects VSD activation in KCNQ1 channels, VSD activation was detected by using voltage clamp fluorometry (VCF) while dynamically depleting the endogenous PIP 2 by expressing and activating the lipid-phosphatase CiVSP."
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"The normal inhibition of the IO state by the KCNE1-induced rotation of the VSD would explain the KCNE1-induced +50 mV GV shift, the slow of current activation kinetics, and the change in Rb /K selectivity by only allowing KCNQ1/KCNE1 channels to open to the AO state at more positive voltages."
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