IndraLab

Statements

USP47 binds RPS2. 16 / 16
3 | 1 12
sparser
"Localization data also showed that interaction between USP47 and RPS2 dissociated during ribosomal stress, as shown in xref a,b."
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sparser
"To verify the interaction of USP47 and RPS2, we performed immunoprecipitation with anti-Flag agarose after overexpressing HA-RPS2 and Flag-USP47."
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sparser
"Consistent with these results, we also found that endogenous RPS2 interacted with endogenous USP47 in cells ( xref b)."
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sparser
"As shown in xref a, USP47 interacted with RPS2 in cells."
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sparser
"Next, we examined the direct interaction between USP47 and RPS2 by a Glutathione S-transferase (GST) pull-down assay using the GST-USP47 477–934 protein that includes the ubiquitin-like (UBL) domain, as the UBL domain is known to mediate protein–protein interaction [ xref ]."
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sparser
"These results indicate that USP47 directly interacts with RPS2."
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reach
"As for USP47, the deficiency of UBLs impaired the binding between USP47 and RPS2, indicating that the UBLs of USP47 were also involved in substrate binding (Cho et al., 2020)."
34604226
sparser
"Ribosomal Stress Inhibits the Interaction between USP47 and RPS2 and thus Increases p53."
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sparser
"On the contrary, the interaction between USP47 and RPS2 was not decreased by the addition of MDM2 ( xref d and xref )."
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sparser
"In addition, ribosomal stress decreases the interaction between RPS2 and USP47, and dissociated RPS2 can then inhibit MDM2 and subsequently lead to p53 stabilization."
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sparser
"USP47 Interacts with RPS2."
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biogrid
No evidence text available
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biogrid
No evidence text available
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biogrid
No evidence text available
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sparser
"Since the upregulation of p53 by ribosomal proteins mainly occurred under ribosomal stress, we compared the interaction between USP47 and RPS2 under normal or ribosomal stress."
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sparser
"As shown in xref a, overexpressed USP47 interacted with endogenous RPS2, but this interaction was decreased under treatment with actinomycin D, which is a ribosomal stress inducer."
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