IndraLab

Statements

OTUB1 binds MSH2. 27 / 27
6 | 8 13
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"Therefore, we conclude that OTUB1 interacts with MSH2 mostly through the central domain."
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biogrid
No evidence text available
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sparser
"A recent mass spectrometry study of MMR protein interactomes revealed a potential interaction between MSH2 and OTUB1 ( xref ), a deubiquitilating enzyme that belongs to the ovarian tumor (OTU) superfamily of predicted cysteine proteases ( xref , xref )."
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sparser
"Here, we demonstrate that OTUB1 interacts with MSH2 to prevent MSH2 from ubiquitination through the noncanonical way."
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sparser
"OTUB1 interacts with MSH2 via its deubiquitylation catalytic center."
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sparser
"To determine whether OTUB1 interacts with MSH2, we expressed Flag-tagged MSH2 (Flag-MSH2) and HA-tagged OTUB1 (HA-OTUB1) in HEK293T cells and performed reciprocal pulldown experiments using Flag agarose and HA magnetic beads."
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sparser
"We found that Flag-MSH2 and HA-OTUB1 pulled each other down ( xref A ), which suggests a specific interaction between MSH2 and OTUB1."
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"Although the interaction between MSH2 and OTUB1 is relatively weak, it is much more specific than the control reactions in both the pulldown and the co-immunoprecipitation assays."
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"GST pull-down assays showed that the FL OTUB1 strongly interacted with MSH2 ( xref D )."
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biogrid
No evidence text available
26752685
sparser
"We show that OTUB1 interacts with the central domain of MSH2 ( xref , E and F )."
33640455
reach
"A recent mass spectrometry study of MMR protein interactomes revealed a potential interaction between MSH2 and OTUB1, a deubiquitilating enzyme that belongs to the ovarian tumor (OTU) superfamily of predicted cysteine proteases."
33640455
reach
"Here, we demonstrate that OTUB1 interacts with MSH2 to prevent MSH2 from ubiquitination through the noncanonical way."
33640455
reach
"OTUB1 interacts with MSH2 via its deubiquitylation catalytic center."
33640455
reach
"To determine whether OTUB1 interacts with MSH2, we expressed Flag tagged MSH2 (Flag-MSH2) and HA tagged OTUB1 (HA-OTUB1) in HEK293T cells and performed reciprocal pulldown experiments using Flag agarose and HA magnetic beads."
33640455
reach
"We found that Flag-MSH2 and HA-OTUB1 pulled each other down (XREF_FIG A), which suggests a specific interaction between MSH2 and OTUB1."
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sparser
"Interested in the potential mechanism of MSH2 protein regulation, Wu et al. found that OTUB1 interacts with MSH2 and increases MSH2 protein stability ( xref )."
38264570
reach
"Although the interaction between MSH2 and OTUB1 is relatively weak, it is much more specific than the control reactions in both the pulldown and the co-immunoprecipitation assays."
33640455
reach
"GST pull-down assays showed that the FL OTUB1 strongly interacted with MSH2 (XREF_FIG D)."
33640455
biogrid
No evidence text available
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biogrid
No evidence text available
19615732
sparser
"We also observed strong interactions between MSH2 and OTUB1(48–271) and OTUB1(48–159), but not between MSH2 and OTUB1(1–47) or OTUB1(159–271) ( xref D )."
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sparser
"Taken together, these results indicate that OTUB1 physically interacts with MSH2 via its middle C1 domain, including its catalytic center."
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sparser
"To determine which MSH2 domain interacts with OTUB1, we first generated GST-tagged full-length MSH2 and three GST-tagged MSH2 mutants: MSH2 (1–378), MSH2 (200–700), and MSH2 (624–934) ( xref E ), as described previously ( xref )."
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sparser
"To determine whether the OTUB1–MSH2 interaction regulates the stability of MSH2, we measured Flag-MSH2 levels in HEK293T cells with or without the overexpression of HA-OTUB1."
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biogrid
No evidence text available
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biogrid
No evidence text available
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