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Statements

Ouabain inhibits JAK1. 11 / 11
| 10 1
eidos
"Therefore , JAK1 diminishment by ouabain is not associated with Na + / K + - ATPase ."
| PMC
eidos
"Ouabain induced JAK1 degradation through ubiquitination mediated proteolysis ."
| PMC
eidos
"Moreover , this Ndfip1 / 2 and NEDD4 mediated JAK1 diminishment by ouabain is endogenous , because ouabain and other natural cardenolides downregulated JAK1 regardless of whether or not the ST or HCT-8 cells were infected by coronaviruses ( Figures 1 & 2 ) ."
| PMC
eidos
"Since Na + / K + - ATPase is the perceptive pharmacological target of ouabain [ 18 ] , [ 19 ] , [ 20 ] , we first dissected whether JAK1 diminishment by ouabain was associated with Na + / K + - ATPase ."
| PMC
reach
"In addition, we also discovered that ouabain also suppressed coronaviral replication by downregulating JAK1 mediated by Ndfip1/2 and NEDD4 E3 ligase via Na+/K+-ATPase-independent proteolysis and that tyrphostin AG1024 (an IR/IGF-1R inhibitor) but not picropodophyllin (PPP) (a selective IGF-1R inhibitor) significantly inhibited TGEV replication by ~84% (residual activity ~16 ± 6%) at a concentration of 10 μM, and enhanced the anti-TGEV activity of ouabain (Table 1)."
35215353
eidos
"A. Ndfip1 / 2 mediated the ouabain induced JAK1 deregulation ."
| PMC
eidos
"Deregulation of JAK1 by ouabain induced proteolysis was mediated by Ndfip1 / 2 and E3 ligase NEDD4 A few ubiquitination proteases and phosphatases have been reported to mediate JAK1 degradation [ 25 ] , [ 26 ] , [ 27 ] , including Ndfip1 / 2 by activation of the proteolysis ligase NEDD4 [ 25 ] ."
| PMC
eidos
"Herein , we knocked down the expression of either Ndfip1 or Ndfip2 ( Figure 6 A ) and found that depletion of either was able to significantly restore the downregulated JAK protein level caused by ouabain treatment ( Figure 6B ) Furthermore , the NEDD4 inhibitor Heclin was also able to significantly rescue the ouabain downregulated JAK1 in a dose dependent manner ( Figure 6C ) ."
| PMC
eidos
"We thus examined these potential effectors and found that Ndfip1 / 2 may mediate the ouabain induced JAK1 deregulation ."
| PMC
eidos
"C. NEDD4 inhibitor Heclin restored the ouabain downregulated JAK1 ."
| PMC
eidos
"JAK1 diminishment by ouabain for mediating TGEV inhibition is independent of Na + / K + - ATPase ."
| PMC