IndraLab

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ChvG activates USP15. 3 / 3
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"The WB and RT-qPCR results all confirmed the successful isolation of Exos overexpressing USP15 from ADMSC.Macrophages, as an important component of the human immune system, are subdivided into M1 and M2 types based on their functional characteristics and surface markers."

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"Using the Annexin V-FITC/PI Apoptosis Detection Kit (Yeasen, Shanghai, China), the effect of USP15-overexpressing Exos on the apoptosis of LPS-treated C28/I2 cells were evaluated by flow cytometry."

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"Finally, flow cytometry analysis was performed with FITC as the x-axis and PI (PE-A) as the y-axis.To investigate the specific role of USP15-overexpressing Exos on LPS-induced polarization of THP1-M0 cells, the following experimental procedures were conducted: Firstly, the USP15-overexpressing Exos were co-cultured with LPS-treated THP1-M0 cells to induce their polarization."