IndraLab

Statements

PRF1 binds STAMBP. 8 / 8
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sparser
"Some residues show a strong signal decrease at an early stage of the titration but it is less pronounced compared to AMSH-P1 (Figs."
27725184
sparser
"We monitored CSPs on a 15 N-UIM-SH3 sample upon titration by AMSH-P1 and made different observations."
27725184
sparser
"The AMSH-P1 peptide, which contains the minimum sequence length for the AMSH-SBM, binds to SH3 with a dissociation constant of 2.0 ± 0.7 μM and a dissociation rate constant ( k off ) of 117 ± 43 s − 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27725184
sparser
"It is also noticeable from our ensemble of titration experiments that the directions of the shifts on UIM-SH3 are similar to that of the complete set of peptides from AMSH-P1 to AMSH-P8."
27725184
sparser
"To address this question, we used the peptide sequence 231 PVVDRSLKP 239 (further denoted by AMSH-P1), known to be a shared STAM-binding motif [7,30] ."
27725184
sparser
"As a consequence, it can be inferred that the mode of binding experienced by AMSH-P1 or Lys63-Ub 2 with SH3 is different."
27725184
sparser
"The kinetic information provided here reveals a residence time of 8.5 ms for AMSH-P1, comparable with other SH3/peptide systems [34,35] and characteristic of a transient interaction."
27725184
sparser
"To address this question, we used different variants of the SBM sequence ( Table 2 ) and the same approach as that for AMSH-P1."
27725184