IndraLab

Statements

OTUD5 binds GST. 3 / 3
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"To test whether OTUD5 also interacts with SPT16 in an unbiased method, we performed FLAG-OTUD5 IP and GST-OTUD5 pulldown experiments from HeLa S3 cells and subjected the eluate to mass spec analysis (Figure xref )."
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"Recombinant GST-OTUD5 WT, but not GST-OTUD5 ΔUIM interacted with SPT16, further confirming that the UIM is necessary for the interaction (Figure xref )."
30508113
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"We thus extended this finding with bacterially purified GST-OTUD5 proteins; both full-length OTUD5 and a C-terminal fragment of OTUD5 (residues 501–571) similarly co-purified SPT16 (Figure xref ), suggesting that the C-terminus is sufficient to bind FACT."
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