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"These results suggest that CaM is produced by the CALM1 gene in the early-stage HDFs with an altered dermal composition, and that they are activated in the biological pathways associated with calcium ions.Since calcium signaling is presumably associated with the differentiation of fibroblasts in the elastin-variable hydrogel environments, we assessed the expression of S100A4 (also called fibroblast-specific protein 1, FSP1), which is a marker of differentiated fibroblasts as well as a calcium-binding protein."