IndraLab

Statements


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"Plasmids encoding Flag-USP37 and HA-Snail were co-expressed in 293T cells."

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"Our studies showed that ectopically expressed Flag-USP37 was able to immunoprecipitate endogenous Snail protein in lung cancer H1299 cells ( xref )."

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"We also expressed GST-Snail in E. coli BL21 cells, which was mixed with Flag-USP37 protein purified from 293T cells, and co-IP assays were carried out with GST beads."

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"HA-Snail and Flag-USP37 were transiently expressed either separated or combined."

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"Endogenous Cdt1 co-immunoprecipitated with overexpressed Flag-USP37 ( Figure 3 A)."

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"Also, overexpression of an siRNA resistant version of Flag-USP37 rescued the drop in Cdt1 levels caused by USP37 depletion ( Supplemental Figure 4A )."