IndraLab

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USP6 activates NFkappaB. 30 / 30
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"Here we demonstrate that TRE17 activates canonical p65-containing NF-κB through an atypical mechanism that is independent of IκB phosphorylation/degradation."
22081069
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"IκB-SR completely abrogated activation of NF-κB by TRE17 as determined by EMSA ( xref )."
22081069
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"We previously reported that USP6 activates NF-kappaB, and that NF-kappaB plays an essential role in USP6 mediated transformation."
27440725
eidos
"In these models , activation of STAT3 , NF-kappaB , and beta-catenin by USP6 was essential for tumorigenesis , as genetic or pharmacologic inhibition of these pathways significantly attenuated xenograft growth ( 16 , 23 , 24 ) ."
33558334
sparser
"In these models, activation of STAT3, NF-κB, and β-catenin by USP6 was essential for tumorigenesis, as genetic or pharmacologic inhibition of these pathways significantly attenuated xenograft growth ( xref , xref , xref )."
33558334
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"We recently reported that TRE17 activates NF-κB, in a manner dependent on its USP activity ( xref )."
22081069
sparser
"Optimal activation of NF-κB by TRE17 requires both catalytic subunits of IKK, distinguishing its mechanism from the classical and non-canonical pathways, which require either IKKβ or IKKα, respectively."
22081069
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"This USP6 dependent activation of NF-kappaB requires IKKalpha, IKKbeta and NEMO in order for p65 S536 phosphorylation to take place."
26999213
sparser
"We recently reported that activation of NF-κB by TRE17 is mediated in part by RhoA and ROCK ( xref )."
22081069
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"Activation of NF-kappaB by USP6 was completely suppressed in these cells (XREF_FIG, left), in accordance with our previous results."
27440725
sparser
"In aggregate these data indicate that TRE17(long) activates classical NF-κB through a mechanism that does not appear to involve phosphorylation and degradation of IκB."
22081069
sparser
"This USP6 dependent activation of NF-κB requires IKKα, IKKβ and NEMO in order for p65 S536 phosphorylation to take place."
26999213
sparser
"In the current study we examine the mechanism by which it functions, demonstrating that TRE17 activates classical NF-κB through an atypical mechanism that is independent of IκB degradation."
22081069
sparser
"However, the mechanism by which TRE17 activates NF-κB and the selective involvement of the classical vs. non-canonical pathways were not examined."
22081069
sparser
"Together, these data suggest that TRE17 activates NF-κB by promoting S536 phosphorylation of p65, in manner that optimally requires IKKα, IKKβ, and NEMO."
22081069
sparser
"We next sought to determine which IKK subunits were required for activation of NF-κB by TRE17."
22081069
sparser
"Activation of NF-κB by USP6 was completely suppressed in these cells ( xref , left), in accordance with our previous results ( xref )."
27440725
sparser
"The findings above suggest that TRE17(long) may activate NF-κB by regulating nuclear translocation of an IκB-free population of p65."
22081069
sparser
"In sum, these data indicate that TRE17(long) selectively activates classical NF-κB, consisting of either homo- or heterodimeric p65 complexes, in three different cell types."
22081069
sparser
"Our finding that TRE17 activates classical NF-κB in the absence of IκB degradation, and in a manner requiring both IKKα and IKKβ, adds to the growing list of stimuli that activate NF-κB through mechanisms distinct from simplified depiction of the classical pathway ( xref ; xref )."
22081069
sparser
"TRE17 activates canonical NF-κB independently of IκB phosphorylation and degradation."
22081069
sparser
"To determine whether TRE17 activates classical or non-canonical NF-κB, electrophoretic mobility shift assays (EMSAs) were performed."
22081069
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"However, only one DUB, TRE17 and USP6, has been shown to induce NF-kappaB activation 27."
30194441
reach
"This validates our cellular model systems, and indicates that USP6 expression in human tumors triggers STAT3 and NF-kappaB signaling responses."
27440725
sparser
"In summary, these studies uncover an unexpected signaling mechanism for activation of classical NF-κB by TRE17."
22081069
sparser
"We previously reported that USP6 activates NF-κB, and that NF-κB plays an essential role in USP6-mediated transformation ( xref , xref )."
27440725
sparser
"Although IκB degradation is not normally elicited by TRE17, we speculated that overexpression of the IκB Super Repressor (IκB-SR) might nevertheless effectively sequester p65 and block NF-κB activation by TRE17(long)."
22081069
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"We next examined whether activation of NF-κB by TRE17 was required for tumorigenesis."
22081069
sparser
"These results indicate that IKKβ, NEMO and, unexpectedly, IKKα all contribute to activation of NF-κB by TRE17(long)."
22081069
sparser
"Since inhibition of RhoA/ROCK only partially attenuated activation of NFκB by TRE17, this suggests that TRE17 functions through additional parallel pathways, in a manner that requires its USP activity."
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