IndraLab

Statements

KCNA3 binds BAX. 29 / 33
2 1 | 9 20
sparser
"Immunoprecipitation and patch-clamp data showed that Bax interacts with the vestibule region of Kv1.3 and induces channel inhibition [33,73] ."
20102714
sparser
"Further studies using recombinant Bax, Bak or Kv1.3 as well as co-immunoprecipitation experiments of Bax and Kv1.3 from isolated mitochondria revealed that Kv1.3 and Bax physically interact upon induc[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25443654
sparser
"Most importantly, patch clamp studies revealed a functional interaction of Kv1.3 with Bax [49,82] ."
25443654
sparser
"Since protonation of Histidine 404 of Kv1.3 has been shown to reduce the interaction of the pore with toxins, it is assumed that Bax binds to and inhibits Kv1.3 in a very similar manner as highly spec[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25443654
sparser
"The strength of this interaction significantly decreased when pH was lowered to 6.0, further confirming that Bax binds to Kv1.3 in a manner similar to Margatoxin."
20102714
sparser
"Strongly relating potassium channels with the regulation of apoptosis, an physical interaction between Bax and Kv1.3 was reported in lymphocytes, which appeared as necessary for Bax-induced apoptosis xref ."
22723988
sparser
"The interaction of Bax and Kv1.3 was pH-dependent and the IC 50 of Bax increased from 4 to 12 nM when the bath solution pH was lowered to pH 6.7 and to a value ≫50 nM at pH 6.0."
25443654
sparser
"This points to an interaction of Bax with the Kv1.3 and the authors showed, indeed, that they physically interact."
20036666
sparser
"Transfection of Bax/Bak double knock-out MEF cells with expression vectors for wildtype Bax, K128E Bax or E158K Bcl-x L confirmed the suggested mechanism of Kv1.3-Bax interactions and showed that tran[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25443654
sparser
"Several further studies demonstrated that Bax does not only interact with Kv1.3, but also with the homologs of Kv1.3, Kv1.5 and Kv1.1 [49,82] ."
25443654
reach
"In lymphocytes, Bax interaction with mitochondrial Kv1.3 is a crucial step that precedes cytochrome c release during apoptosis (Gulbins et al., 2010)."
38292023
sparser
"In this mechanism, Kv1.3 on the mitochondrial membrane interacts with Bax (Bcl-2 family), triggering apoptosis in the way of toxoid ( xref )."
34093186
sparser
"Our results point to a physiologically relevant interaction of Bax with Kv1.3 and further indicate a crucial role of a distinct lysine in determining the proapoptotic character of Bcl2-family proteins."
20885444
reach
"These analogies between Kv1.3 toxins and Bax led to studies that tested whether Bax and Bak bind to Kv1.3 and induce cell death in a toxin-like fashion."
25443654
reach
"The interaction of Bax and Kv1.3 was pH-dependent and the IC 50 of Bax increased from 4 to 12 nM when the bath solution pH was lowered to pH 6.7 and to a value ≫50 nM at pH 6.0."
25443654
reach
"Since protonation of Histidine 404 of Kv1.3 has been shown to reduce the interaction of the pore with toxins, it is assumed that Bax binds to and inhibits Kv1.3 in a very similar manner as highly spec[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25443654
sparser
"Bax binds to Kv1.3 and functionally inhibits mitochondrial Kv1.3 [ xref , xref ]."
35454865
sparser
"The strength of this interaction significantly decreased when pH was lowered to 6.0, confirming again that Bax binds to Kv1.3 in a manner similar to Margatoxin."
20114030
reach
"The strength of this interaction significantly decreased when pH was lowered to 6.0, confirming again that Bax binds to Kv1.3 in a manner similar to Margatoxin."
20114030
sparser
"To further prove the specificity of the interaction of Bax and Kv1.3 we added MgTx (5 μM) to GST-Kv1.3 (∼ 2 nM) prior to addition of GST-Bax."
20114030
sparser
"As MgTx specifically binds to the pore of Kv1.3 with very high affinity (IC 50 : 110 pM), an excess of MgTx (5 μM) will prevent binding of Bax to Kv1.3, but only if Bax binds in the pore region of Kv1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20114030
reach
"To further prove the specificity of the interaction of Bax and Kv1.3 we added MgTx (5 μM) to GST-Kv1.3 (∼ 2 nM) prior to addition of GST-Bax."
20114030
reach
"As MgTx specifically binds to the pore of Kv1.3 with very high affinity (IC 50 : 110 pM), an excess of MgTx (5 μM) will prevent binding of Bax to Kv1.3, but only if Bax binds in the pore region of Kv1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20114030
sparser
"In lymphocytes, Bax interaction with mitochondrial Kv1.3 is a crucial step that precedes cytochrome c release during apoptosis ( xref )."
38292023
sparser
"We have also shown that Bax binds to the pore of Kv1.3, subsequently resulting in the inhibition of Kv1.3 activity in isolated mitochondria."
22496117
reach
"Bax binds to Kv1.3 and functionally inhibits mitochondrial Kv1.3 [17,18]."
35454865
sparser
"Although our data indicate PTP activation downstream of Bax–Kv1.3 interaction, we should stress that they do not discriminate whether cytochrome c efflux is directly linked to PTP opening – which may [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20114030
reach
"The strength of this interaction significantly decreased when pH was lowered to 6.0, further confirming that Bax binds to Kv1.3 in a manner similar to Margatoxin."
20102714
sparser
"To gain insight into the mechanism of Bax-Kv1.3 interaction, we mutated Glu158 of Bcl-x(L) (corresponding to K128 in Bax) to lysine."
20885444