IndraLab

Statements

sparser
"The covalent complex between Josephin-2 and ubiquitin mimics the covalent thioester intermediate formed during the deubiquitination reaction ( xref )."
32647816
sparser
"Additionally, in the covalent complex of JOSD2 and ubiquitin, the ubiquitin molecule is covalently attached to cysteine-24, which moves side chain of this residue away from histine-125 and toward the binding site of the substrate."
32982735
sparser
"The crystal asymmetric unit contains a single copy of the ubiquitin-Josephin-2 complex."
32647816
sparser
"In order to form a Josephin-2-ubiquitin complex sufficiently stable for crystallization, we covalently linked the enzyme to its substrate using ubiquitin 1–75 -chloroethylamine, an active site-directed ubiquitin analog ( xref )."
32647816