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Sal-miR-1 inhibits OTUD7B. 10 / 10
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"Sal-miR-1 and 3 abrogate OTUD7B upregulation by thrombin via targeting the OTUD7B 3 ' UTR."
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"Most importantly , OTUD7B downregulation by Sal-miR-1 and 3 attenuates KLF4 protein levels via decreasing its deubiquitylation , whereas decreased KLF4 relieves its repression of transcription of NMHC IIA gene and thus increases NMHC IIA expression levels ."
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"Mechanistically, Sal-miR-1 and 3 abrogate OTUD7B upregulation by thrombin via binding to the different sites of the OTUD7B 3 ' UTR."
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"Sal-miR-1 and 3 abrogate OTUD7B upregulation by thrombin via targeting the OTUD7B 3'UTR Because plant-derived miRNAs are known to be capable of regulating mammalian gene expression by binding to the 3 ' UTR of target genes 19 , 29 , we utilized the bioinformatic databases miRanda ( http://www.microrna.org/microrna/home.do ) , Targetscan ( http://www.targetscan.org/vert_72/ ) and RNAhybrid ( https://bibiserv.cebitec.uni-bielefeld.de/rnahybrid/ ) to predict Sal-miR-1 and 3 targets ( Figure S3A , Table S1 ) ."
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"OTUD7B downregulation by Sal-miR-1 and 3 attenuates KLF4 protein levels To clarify which substrate is regulated and deubiquitinated by OTUD7B in the context of VSMC migration and adhesion , we used 3 protein-protein interaction prediction databases ( Cytoscape : http://apps.cytoscape.org/apps/agilentliteraturesearch; inBio-Map : https://www.intomics.com/inbio/map.html#search; Genemania : http://genemania.org ) to search for putative proteins interacting with OTUD7B ."
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"Mechanistically , Sal-miR-1 and 3 abrogate OTUD7B upregulation by thrombin via binding to the different sites of the OTUD7B 3'UTR ."
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"We show here that OTUB7B upregulates the level of KLF4 protein , a stem cell pluripotency protein 41 , through interacting with and deubiquitylating KLF4 , a repressor of VSMC differentiation , whereas OTUD7B downregulation by Sal-miR-1 and 3 attenuates KLF4 protein level ."
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"Results of luciferase reporter assays using OTUD7B 3'UTR or its mutant plasmids demonstrated that Sal-miR-1 and 3 could target the 3'UTR of OTUD7B mRNA and then inhibited OTUD7B expression ."
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"Importantly , OTUD7B downregulation by Sal-miR-1 and 3 results in a reduction in KLF4 protein level , which in turn relieves KLF4 repression of the expression of SM alpha-actin , SM22alpha , and SMMHC , thus facilitating VSMC contractile gene ( VSMC differentiation gene ) expression ."
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"In this study, our results showed that Sal-miR-1 and 3 reduced the upregulation of the OTUD7B mRNA and protein induced by thrombin."
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