IndraLab

Statements

Piwil1 binds EIF3F. 6 / 6
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sparser
"We confirmed the interaction between MIWI and eIF3f by co-immunoprecipitation (coIP) in both co-transfected 293T cells ( xref ) and mouse testes ( xref )."
31835033
sparser
"To begin to determine whether MIWI-binding to eIF3f is required for translation activation of piRNA targets in mouse spermatids, we mapped the domain(s) in MIWI responsible for eIF3f-binding by testing a series of MIWI truncation mutants ( xref ) for coIP with full-length eIF3f."
31835033
sparser
"We found that a 150-aa (amino acid) MIWI N-terminal peptide (MIWI-N2) efficiently interacted with eIF3f and deletion of 10 aa at position 111–120 (MIWI-N2 Δ111–120 ) abolished its binding with eIF3f ( xref )."
31835033
sparser
"Importantly, MIWI-N2, but not MIWI-N2 Δ111–120 , effectively impaired the binding of full-length MIWI to eIF3f ( xref )."
31835033
sparser
"This indicates that the association of MIWI with eIF3f is temporally regulated during spermatogenesis."
31835033
sparser
"Interestingly, while the interaction between MIWI and eIF3f and the interaction between HuR and eIF4G3 are both independent of RNA, the formation of the MIWI/eIF3/HuR/eIF4G3 super-complex is RNA mediated."
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