IndraLab
Statements
reach
"To select mutants that are defective in the erg3 dependent induction of DAN1, we mutagenized erg3 ura3 cells harboring the DAN1-URA3 plasmid with ethanemethanesulfonate, allowed the cells to recover from the mutagenesis, and then selected for cells that grew on geneticin containing 5-FOA plates (thus defective for DAN1 induction)."