IndraLab

Statements


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sparser
"Moreover, recombinant GST-OTUD1 completely hydrolyzed the K63-linked polyubiquitin chain, and processed a long K48-linked polyubiquitin chain to tetraubiquitin (Supplementary Fig. xref )."

sparser
"To ensure that OTUD1 interacts directly with Akt, we further constructed and purified GST-OTUD1 and His-Akt from E . coli ( Figure S1 C), and pulldown assays were performed."

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"An in vitro pulldown assay with purified GST-OTUD1 and His-PH demonstrated that the Akt PH domain directly bound to OTUD1 ( Figures 3 D and S3 C)."