IndraLab

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MicroRNAs decreases the amount of USP32. 5 / 5
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"As a result, miR let-7a might suppress the expression of USP32 protein by directly binding to the USP32 3’UTR."
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"Western blot analysis revealed that miR let-7a mimics dramatically decreased the USP32 protein expression, whereas miR let-7a inhibitors increased the protein expression of USP32 compared with their controls in the MCF-7 cells."
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"There has been no data showing the correlations between the expression of USP32 and miRNA.To validate whether USP32 protein expression is downregulated by miR let-7a, we examined the level of USP32 protein after transfecting miR let-7a mimics and inhibitors."
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"Taken together, these results confirmed that miR let-7a can inhibit USP32 protein expression level by directly binding USP32 3'UTR.Our present results showed that USP32 expression is up-regulated in human BCa cells and miR let-7a significantly inhibits USP32 expression."
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"Collectively, the results demonstrated that miR let-7a could downregulate the protein levels of USP32 in MCF-7 cells."
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