IndraLab

Statements

SKP2 binds USP18. 31 / 31
6 | 15 10
reach
"Hence, expression of ISG15 abrogates the USP18 and SKP2 complex, and this results in destabilization of SKP2 and accumulation of USP18."
30858391
sparser
"Interestingly, USP18 binds to SKP2, an interaction partner of p21 and also precipitates SAMHD1 [ xref ]."
38675828
sparser
"Also USP18 interacted with S-phase kinase associated protein 2 (SKP2), retained cylin A and down regulated p21 in differentiated THP-1."
| PMC
sparser
"The association of ISG15 with USP18 interrupts the interaction of USP18 with S-phase kinase-associated protein 2 (SKP2), inhibiting the proteasomal degradation of USP18, which is essential for negative feedback regulation of IFN signaling and prevention of autoinflammation xref , xref ."
36319753
sparser
"Several studies have reported that SAMHD1 interacts with cyclin/CDK complexes [ xref ], USP18 and S-phase kinase-associated protein 2 [ xref , xref ], which are involved in the regulation of cell proliferation [ xref , xref ] and SAMHD1 is also recruited to DNA repair foci in response to DNA damage [ xref ]."
37667113
sparser
"Numerous studies have reported that SAMHD1 can interact with cyclin/CDK complexes ( xref ), USP18 ( xref ) and S-phase kinase-associated protein 2 ( xref ), which are involved in the regulation of cell proliferation ( xref , xref )."
33907561
reach
"Along with others, we reported that SKP2 interacts with USP18 and promotes its degradative ubiquitination XREF_BIBR, XREF_BIBR."
30858391
reach
"We found that USP18 and SKP2 interact and that free ISG15 abrogates the complex, liberating USP18 from degradation and concomitantly driving SKP2 to degradation and/or ISGylation."
30858391
sparser
"While these data do not rule out the possibility that indirect interactions between ISG15 (via Trp123) and an unknown partner are responsible for USP18 stability, which may be consistent with previous reports showing that ISG15 can abrogate the USP18‐SKP2 complex and rescue USP18 from proteasomal degradation independently of its ability to bind USP18 [ xref ]."
39931755
biogrid
No evidence text available
25307056
reach
"ISG15 interferes with the USP18 and SKP2 complex."
30858391
reach
"In a first set of experiments, we analyzed the impact of ISG15 on the USP18 and SKP2 complex."
30858391
reach
"In this condition the USP18 and SKP2 complex was more abundant and yet expression of ISG15 abrogated it (lane 2 vs lane 4)."
30858391
reach
"As additional control, we co-transfected STAT2, another USP18 interactor 21, which did not interfere with the USP18 and SKP2 complex."
30858391
reach
"Overall, these results indicate that free ISG15 interferes with the USP18 and SKP2 complex regarless of the catalytic activity of USP18 or its ISG15 binding potential."
30858391
reach
"Overall, our results indicate that free ISG15, regardless of its conjugation potential, interferes with the USP18 and SKP2 complex through a mechanism that does not require direct binding to USP18 or ISGylation/de ISGylation events."
30858391
sparser
"We found that USP18 and SKP2 interact and that free ISG15 abrogates the complex, liberating USP18 from degradation and concomitantly driving SKP2 to degradation and/or ISGylation."
30858391
reach
"Although this is in line with the view that only a small fraction of the total pool of a given protein is ISGylated 26, we inferred that low levels of SKP2 ISGylation is unlikely to account neither for the disruption of the USP18 and SKP2 complex nor for the reduction of SKP2 (47kDa) observed upon enforced ISG15 expression."
30858391
biogrid
No evidence text available
30858391
sparser
"By co-immunoprecipitation we confirmed the binding of exogenous SKP2 (55 kDa) to USP18 (Fig.  xref , lane 1)."
30858391
reach
"Interestingly, ISG15 not only abrogates the USP18 and SKP2 complex, but also renders SKP2 more prone to degradation."
30858391
sparser
"Along with others, we reported that SKP2 interacts with USP18 and promotes its degradative ubiquitination xref , xref ."
30858391
reach
"As we found that USP18 formed a complex with the E3 ubiquitin ligase recognition factor S-phase kinase associated protein 2 (SKP2) and SAMHD1, we initially assumed that the protein stability or ISGylation of SAMHD1 would affect the antiviral activity of SAMHD1 [124]."
38675828
reach
"Interestingly, USP18 binds to SKP2, an interaction partner of p21 and also precipitates SAMHD1 [124]."
38675828
reach
"The formation of the USP18 and SKP2 complex (XREF_FIG, lane 8), was prevented by co-expression of ISG15 (lane 10 versus lane 8) and ISG15 co-immunoprecipitated with USP18 independently of SKP2 expression (lanes 9 and 10)."
25307056
reach
"USP18 formed a complex with the E3 ubiquitin ligase recognition factor SKP2 (S-phase kinase associated protein 2) and SAMHD1."
30068654
biogrid
No evidence text available
15342634
biogrid
No evidence text available
15342634
biogrid
No evidence text available
15342634
sparser
"It has been reported that ISG15 has a role in regulating the cell cycle through its interactions with SKP2 and USP18, although experiments in that study were not performed in IFN-treated cells, nor were ISG15 knockout cells tested ( xref )."
32423918
biogrid
No evidence text available
30068654