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"We performed western blot analysis on cellular extracts obtained from both siUSP48-transfected cells and control cells, stimulated with TNFα at different time points, and found that USP48 downregulation slightly increased PCNA expression (indicative of an NF-κB positive regulation), and reduced the expression of Cyclin E (indicative of an NF-κB negative regulation), in no TNFα-treated cells."