IndraLab

Statements

EGFP binds CALM1. 4 / 4
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"We used dual-color TIRF-SIM imaging to investigate the spatial distribution of AP2 and CALM adaptors within clathrin-coated pits and plaques that form in SUM-159 cells that are genome edited to expres[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Recombinant Agrobacterium tumefaciens GV3101 strains harboring either the StMAPK19-EGFP fusion expression vector (pCAM35S-EGFP-StMAPK19) or the empty vector control (pCAM35S-EGFP) were separately infiltrated into tobacco leaves via syringe injection."
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"Similar results were obtained when clathrin-coated pit images from SUM-CALM cells were analyzed: the FWHM was 125 ± 25 nm for CALM-EGFP and 171 ± 19 nm for mRuby-clathrin ( N structures = 79)."
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"Notably, GFP signals from the empty vector control (pCAM35S-EGFP) were also diffusely distributed across the cell membrane, cytoplasm, and nucleus."
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