IndraLab

Statements


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"Consistent with this, ectopically expressed Flag-USP35 was associated with endogenous Aurora B in HEK293T and HeLa cells, and conversely, endogenous USP35 was precipitated efficiently by HA-Aurora B affinity purification (Supplementary Fig.  xref , xref , c)."

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"To test whether Ser613 can be phosphorylated by AKT, Flag-USP35 WT or Flag-USP35 S613A vector was cotransfected with the activated AKT mutant plasmid into 293 T cells."

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"To explore the effects of Ser613 phosphorylation on USP35 localization and function, MCF-7 and T-47D cells were engineered to express vector alone, Flag-USP35 WT , Flag-USP35 S613A , and Flag-USP35 S613E (Fig. xref )."