IndraLab

Statements

USP35 binds Flag. 4 / 4
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"To explore the effects of Ser613 phosphorylation on USP35 localization and function, MCF-7 and T-47D cells were engineered to express vector alone, Flag-USP35 WT , Flag-USP35 S613A , and Flag-USP35 S613E (Fig. xref )."
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"Consistent with this, ectopically expressed Flag-USP35 was associated with endogenous Aurora B in HEK293T and HeLa cells, and conversely, endogenous USP35 was precipitated efficiently by HA-Aurora B affinity purification (Supplementary Fig.  xref , xref , c)."
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"Similarly, when Flag-USP35 and HA-Snail1 vectors were transfected into HEK293T and GC cells, HA-tagged Snail1 can also co-immunoprecipitate with Flag-tagged USP35 upon addition of a Flag antibody (Fig. xref D)."
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"To test whether Ser613 can be phosphorylated by AKT, Flag-USP35 WT or Flag-USP35 S613A vector was cotransfected with the activated AKT mutant plasmid into 293 T cells."
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