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GRHL3 binds USP39. 16 / 16
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"These findings further support the idea that endogenous USP39 might bind GRHL3 in living cells."
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"To test the interaction between GRHL3 and USP39 within MCF7 cells under physiological conditions, we immunoprecipitated protein complexes in cell extracts using an antibody against GRHL3."
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"To verify the interaction between USP39 and GRHL3 proteins, a visible immunoprecipitation (VIP) assay, a simple method to examine the interaction between two proteins without respective antibodies, was performed (Fig. S xref ) xref ."
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"This finding suggests the possible interaction between GRHL3 and USP39 in vitro."
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"To map which protein domains are responsible for the interaction between GRHL3 and USP39, we generated two series of constructs consisting of three domains of Grhl3 cDNA and two domains of Usp39 cDNA fused to GST , respectively, and examined binding activity to each other (Fig. S xref )."
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"These biochemical studies demonstrate that over-expressed GRHL3 can interact with USP39 via the CP2/Ub-like domains of GRHL3, and the ZnF domain of USP39."
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"These aforementioned findings, together with biochemical results, suggest that over-expressed USP39 interacts with GRHL3 in the cytoplasm mainly through the Ub-like domain of GRHL3."
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"To evaluate the genetic interaction between Grhl3 and Usp39 , we analyzed phenotypes of epidermal development during eyelid closure since Grhl3 −/− embryos have been reported to be defective in eyelid closure with a low penetrance (Figs.  xref and S xref ) xref , xref ."
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"To explore the exact timing and place of GRHL3 interaction with the USP39 protein in epidermal cells, we analyzed expression of the two genes and eyelid phenotypes more closely (Fig.  xref )."
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"To determine when Grhl3 interacts with Usp39 during eyelid closure, we examined Grhl3 and Usp39 expression at the mRNA level in sections, and at the protein level in whole-mount of wild-type embryos at E15.5 (Figs.  xref and S xref )."
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"These results support the notion that GRHL3 can interact with USP39 under physiological conditions."
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"To test the interaction between GRHL3 and USP39 within MCF7 cells under physiological conditions, we immunoprecipitated protein complexes in cell extracts using an antibody against GRHL3."
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"This finding suggests the possible interaction between GRHL3 and USP39 in vitro.To confirm if GRHL3 localized in close proximity to USP39 in living cells, we analyzed protein–protein interaction using fluorescent protein fragments of a monomeric Kusabira-Green reporter system (mKG; CoralHueR Fluo-chase Kit) in MCF7 and NIH3T3 cells (Fig. 1a, b)."
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"These findings further support the idea that endogenous USP39 might bind GRHL3 in living cells.To map which protein domains are responsible for the interaction between GRHL3 and USP39, we generated two series of constructs consisting of three domains of Grhl3 cDNA and two domains of Usp39 cDNA fused to GST, respectively, and examined binding activity to each other (Fig. S2b–e)."
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"To evaluate the genetic interaction between Grhl3 and Usp39, we analyzed phenotypes of epidermal development during eyelid closure since Grhl3 embryos have been reported to be defective in eyelid closure with a low penetrance (Figs."
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"Usp39 interacts genetically with Grhl3 primarily through non-canonical Wnt signaling for epithelial morphogenesis."
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