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USP25 increases the amount of NANOG. 7 / 7
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"Through the screen they also found that USP21 significantly upregulated Nanog levels while other DUBs had little to no effect on the Nanog expression levels."
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"Knockdown of circCD2AP or USP21 inhibited the expression of USP21, OCT4, Nanog, Sox2, and ALDH1A1 in tumor tissues (Figures 7D–7H), suggesting the inhibiting effects of circCD2AP or USP21 knockdown on cancer cell stemness."
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"Overexpression of USP21, but not the catalytically inactive mutant C221A, led to increased Nanog levels in a dose dependent way (XREF_FIG), suggesting that USP21 upregulation of Nanog expression is dependent on DUB enzyme activity."
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"Overexpression of wild-type USP21, but not the catalytically inactive form of USP21 (C221S), led to increased expression level of NANOG ( Fig. 4 B)."
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"The prolyl isomerase Pin1 and deubiquitinase USP21 have been suggested to stabilize Nanog in ESCs by preventing its degradation through the UPS 18,21-23. Immunoblotting analysis revealed that ectopic expression of USP21, but not Pin1, significantly enhanced the protein level of Nanog in HuH7 cells (Figure 7A)."
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"Co-immunoprecipitation assays showed that N-domain and H-domain of Nanog were required for its interaction with KLF15 (Fig. 4K)Furthermore, rescue experiments were performed in PDAC cells when USP21 is co-overexpressed with KLF15, western blot assay showed in double overexpressed KLF15- USP21 cells, the complement of USP21 restored the expression of Nanog (Fig. S6A-B)."
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"Through the screen, we found that USP21 significantly upregulated Nanog levels, whereas other DUBs had little to no effect on the Nanog expression levels (XREF_FIG)."
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