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MYC binds USP39. 17 / 17
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"Finally, Boyden chamber assays demonstrated that OPM2 and U266 cells overexpressing either the GFP-USP39 or Myc-USP39 form exhibited enhanced migratory capacity."
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"Injection of OPM2 cells stably overexpressing USP39 (Myc-USP39) or control cells (Myc) into the perivitelline space of zebrafish larvae allowed for real time visualization of local and distant metastatic events using fluorescence microscopy after 2 days."
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"Moreover, an in vitro deubiquitination assay designed by mixing USP39-Myc or USP14-Myc with E-HA, which was purified from cells transfected with USP-Myc using anti-Myc beads or cells transfected with [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"We next constructed a series of expression vectors encoding Cyclin B1 mutants in which lysine (K) residues that might be ubiquitinated were replaced with arginine (R), then assessed their ubiquitination levels affected by Myc-USP39 in HEK293T cells."
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"Among the Cyclin B1 mutants we constructed, only the ubiquitination of K242R mutant was not reduced by the Myc-USP39 ( xref h)."
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"USP39 or USP14 was immunoprecipitated from HEK293T cells overexpressing USP39-Myc or USP14-Myc, using anti-Myc antibody plus protein G agarose beads."
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"USP39-Myc failed to co-immunoprecipitation mutant E with a deletion of amino acids 11–26 ( Fig. 4 F) which located in the first helix of the E protein ( Fig. 4 E), and showed weaker interaction with E[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Transfection of a plasmid encoding an exogenous form of USP39 (Myc-USP39) alone did not affect cellular metabolism."
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"However, co-transfection of USP39 siRNA and Myc-USP39 plasmid led to a partial rescue of the decline in cellular metabolism, thus demonstrating the specificity of the observed effects to USP39 modulation (Fig.  xref E)."
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"OPM2 and KMM1 cells were stably transfected with lentiviral vectors encoding either Myc or Myc-USP39 (Fig. S3A and S3C)."
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"To determine whether overexpression of USP39 could impact the sensitivity of myeloma cells to BTZ, we used U266 (Fig. S4A) and KMM1 (Fig. S4B) cells stably transfected with lentiviral particles encoding Myc or Myc-USP39."
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"Furthermore, to investigate the form of ubiquitination chains linked to Cyclin B1, HEK293T cells co-transfected with Flag-CycB1, Myc-USP39 and each one of the different ubiquitins (HA-tagged K6, K11, K27, K29, K33, K48 or K63-ubiquitin, individual ubiquitin constructs could only form ubiquitin linkages at a single lysine) were performed a co-immunoprecipitation assay using anti-Flag-M2 beads."
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"KMM1 cells were transfected with HA-ubiquitin plasmid in the presence or absence of Myc-USP39 plasmid (Fig.  xref G) treated with MG132 to inhibit proteasomal degradation of HA-ubiquitin proteins."
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"Immunoprecipitation of endogenous ZEB1 followed by immunoblotting with HA and Myc antibodies revealed transfected poly-HA-Ubiquitin and exogenous Myc-USP39, respectively (left part)."
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"Furthermore, to reinforce our findings, U266 and OPM2 cells were stably transfected with lentiviral particles encoding either GFP or GFP-USP39 (Fig.  xref C-D) or Myc and Myc-USP39 (Fig.  xref E-F)."
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"The result showed that compared to other HA-Kn-Ubs, the expression level of HA-K29-Ub was downregulated by adding the Myc-USP39 plasmid in the cells ( xref c)."
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