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CTNNB1 binds UCHL3. 16 / 16
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"These results suggest that UCHL3 might physically interact with CTNNB1 to trigger activation of the Wnt signaling pathway."
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"The interaction between UCH-L3 and β-catenin proteins was confirmed, and it reduced the ubiquitination of β-catenin."
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"Hence, we evaluated the interaction between UCHL3 and CTNNB1."
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"A Pierce Co-Immunoprecipitation Kit (Thermo Fisher, USA) was used to verify the physical interaction between UCHL3 and CTNNB1."
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"Interestingly, we found that UCHL3 interacted with CTNNB1, as shown by co-IP and colocalization assays with T24 cells."
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"To verify the interaction between UCHL3 and CTNNB1 in bladder cancer cells, the T24 cell line was selected for an endogenous immunoprecipitation (IP) analysis."
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"UCHL3 interacts with and stabilizes CTNNB1."
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"The results showed that UCHL3 binds endogenous CTNNB1 under pathological conditions (Fig.  xref B)."
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"The interaction between UCH-L3 and β-catenin proteins was observed, resulting in reduced ubiquitination and increased protein stability of β-catenin."
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"Hence, we evaluated the interaction between UCHL3 and CTNNB1."
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"To verify the interaction between UCHL3 and CTNNB1 in bladder cancer cells, the T24 cell line was selected for an endogenous immunoprecipitation (IP) analysis."
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"The results showed that UCHL3 binds endogenous CTNNB1 under pathological conditions (Fig. 4B)."
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"To evaluate the effect of UCHL3 DUB activity on CTNNB1 stability, we overexpressed FLAG-UCHL3 or the FLAG-UCHL3 mutant and found that the stability of FLAG-UCHL3 binding to HA-CTNNB1 depended on its ubiquitinase activity (Fig. 4E)."
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"We further evaluated the interaction of UCHL3 with CTNNB1 using a cycloheximide-chase (CHX) assay to verify that CTNNB1 protein levels are regulated by UCHL3 over time."
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