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USP47 deubiquitinates TLE3. 4 / 4
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"USP47 deubiquitylates TLE3 in cells and in vitro."
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"To more directly determine whether USP47 deubiquitylates TLE3, we performed an in vitro ubiquitylation assay with ubiquitylated TLE3 and recombinant USP47 protein."
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"USP47 decreased the amount of ubiquitylated TLE3 in vitro in a dose-dependent manner (Fig. 6B)."
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"To test how deubiquitylation of TLE3 by USP47 alters TLE3 binding to TCF, we performed an in vitro binding assay and found that TLE3 was pulled down with TCF4, but ubiquitylated TLE3 was not (Fig. 6C), consistent with our previous results (7)."
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