IndraLab

Statements

OTUD3 binds PLK1. 10 / 10
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"These results indicate that OTUD3 interacted with PLK1, and PLK1‐T2 (36–305) is the sequence of PLK1 that binds to OTUD3."
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"We next used co‐immunoprecipitation and western blot methods to analyse the specific fragments of OTUD3 and PLK1 that bind to each other."
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"To explore the regions responsible for the interactions between OTUD3 and PLK1, we generated a set of deletion mutants of PLK1."
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"In previous studies, PLK1 was shown to be involved in ferroptosis in tumour cells and played a key role in promoting tumour cell growth. xref We predicted the binding of OTUD3 and PLK1 proteins using molecular docking techniques."
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"In this study, we found that the PBD domain of PLK1 binds OTUD3."
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"Binding of OTUD3 to PLK1."
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"In vitro pull-down assays with purified recombinant proteins showed that Myc-PLK1 directly bound GST-OTUD3, instead of GST control (Fig. 5G)."
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"To explore the regions responsible for the interactions between OTUD3 and PLK1, we generated a set of deletion mutants of PLK1."
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"Dysregulation of its activity not only directly leads to chromosomal segregation errors and micronucleus formation but may also exacerbate oncogenic risk by interfering with DNA damage repair pathways, making it a highly promising molecular target in cancer therapy. xref , xref , xref Co‐immunoprecipitation and mass spectrometry showed that OTUD3 interacted with PLK1 and then truncated PLK1 into three parts."
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"Ectopically expressed HA-tagged OTUD3 also interacted with Myc-tagged PLK1 in HEK-293T cells, and vice versa (Fig. xref and Supplementary Fig. xref )."
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