IndraLab

Statements

KCNH1 binds E2F1. 7 / 11
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"In proliferating cells, E2F1 transcription factor binds directly to the Kv10.1 promoter during (or close to) G2/M, resulting in transient expression of the channel."
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"To validate whether E2F1 directly binds to the Kv10.1 promoter in vivo , we conducted ChIP experiments pulling down E2F1 in synchronized HeLa cells, and checked the presence of the endogenous Kv10.1 promoter region covering the predicted E2F1 binding site using qRT-PCR on the immunoprecipitated fraction."
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"To test whether Kv10.1 expression is under the influence of the cell cycle through the pRb/E2F1 pathway, we abolished the binding of the E2F1 transcription factor to the promoter region of Kv10.1, reduced the pRb suppression on E2F1 through HPV-E7 overexpression, and examined the consequences in the Kv10.1 promoter activity as well as protein expression in thymidine synchronized HeLa cells."
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"E2F1 preferentially binds to Kv10.1 promoter toward G2/M, resulting in restricted expression of the channel during a short time window in G2/M in cancer cells, and interestingly also in normal proliferating cells."
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"Interaction of E2F1 with the Kv10.1 promoter occurs during G2/M."
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"The interaction between E2F1 and the Kv10.1 promoter was significantly increased when the cells reached the G2/M transition."
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"Transient Kv10.1 expression is transcriptionally induced during G2/M transition by the direct binding of E2F1 to the Kv10.1 promoter [ xref ]."
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