IndraLab

Statements

E2F1 binds KCNH1. 7 / 11
| 7
sparser
"E2F1 preferentially binds to Kv10.1 promoter toward G2/M, resulting in restricted expression of the channel during a short time window in G2/M in cancer cells, and interestingly also in normal proliferating cells."
27029528
sparser
"Transient Kv10.1 expression is transcriptionally induced during G2/M transition by the direct binding of E2F1 to the Kv10.1 promoter [ xref ]."
30823672
sparser
"In proliferating cells, E2F1 transcription factor binds directly to the Kv10.1 promoter during (or close to) G2/M, resulting in transient expression of the channel."
27029528
sparser
"Interaction of E2F1 with the Kv10.1 promoter occurs during G2/M."
27029528
sparser
"To test whether Kv10.1 expression is under the influence of the cell cycle through the pRb/E2F1 pathway, we abolished the binding of the E2F1 transcription factor to the promoter region of Kv10.1, reduced the pRb suppression on E2F1 through HPV-E7 overexpression, and examined the consequences in the Kv10.1 promoter activity as well as protein expression in thymidine synchronized HeLa cells."
27029528
sparser
"The interaction between E2F1 and the Kv10.1 promoter was significantly increased when the cells reached the G2/M transition."
27029528
sparser
"To validate whether E2F1 directly binds to the Kv10.1 promoter in vivo , we conducted ChIP experiments pulling down E2F1 in synchronized HeLa cells, and checked the presence of the endogenous Kv10.1 promoter region covering the predicted E2F1 binding site using qRT-PCR on the immunoprecipitated fraction."
27029528