IndraLab

Statements

USP16 binds mCherry. 3 / 3
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"To determine whether the impaired ZGA was directly caused by the absence of H2AK119ub1 deubiquitination after Usp16 deletion, we overexpressed mCherry-USP16 in fully grown Usp16 -null oocytes by mRNA microinjection."
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"In a rescue experiment, ectopic expression of mCherry-USP16 in Usp16 -null oocytes by mRNA microinjection could not remove H2AK119ub1 from the chromosomes at GV stage, as we speculated ( xref ), but could at MI stage (Figure xref – xref )."
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"Cysteine-205 of USP16 was vital for its deubiquitinase activity; as a negative control, when mCherry-USP16 C205S was expressed in Usp16 -null oocytes, it failed to remove H2AK119ub1 from the chromosomes (Figure xref – xref ), indicating that the deubiquitinase activity of USP16 is essential for H2AK119ub1 removal in maturing oocytes."
35640597