IndraLab

Statements

VCPIP1 increases the amount of X. 4 / 4
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"Both the full-length VCPIP1 and its C-terminal aa 863 to 1221 upregulated the HBx expression induced by plasmid containing replication-competent full-length HBV (Fig. 2E and F)."
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"Functionally, ectopic VCPIP1 expression increased HBx expression in a dose-dependent manner and in the context of HBV infection."
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"To determine whether the increased HBx expression by VCPIP1 was due to the decreased ubiquitination, we performed an in vivo ubiquitination assay by detecting the ubiquitination of HBx proteins using the Flag antibody-conjugated agarose beads to precipitate the HBx."
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"More importantly, it was clearly shown that siPSMC3 treatment greatly impaired the HBx expression increased by VCPIP1 overexpression compared to the siRNA control (Fig. 6F)."
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