IndraLab

Statements

CALM1 binds Myo1. 9 / 9
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sparser
"The rapid, single-molecule, interactions of Myo1 and Cam1 with the membrane had low mobility (0.03 µm 2 ."
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sparser
"To correlate Myo1-Cam1 dynamics at sites of endocytosis with the internalization of the mature endosome into the cytoplasm, we followed Cam1 and Cam2 dynamics simultaneously in cam1."
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sparser
"These conditions favor interactions between Myo1 and Cam2 over an association of Cam1 with Myo1 ( xref ), which is consistent with important roles for both Myo1 and Cam2 at the leading edge of forespore membrane formation during meiosis ( xref ; xref )."
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sparser
"Calcium levels within log phase yeast cells are relatively low (100–200 nM) ( xref ; xref ), and so provide conditions that favor the association of Cam1 with Myo1 (pCa, 5.87)."
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sparser
"These relative protein levels, binding affinities and low Ca 2+ concentrations favor Cam1 binding to Myo1, over Cam2 at both IQ sites ( xref ), consistent with in vivo observations."
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sparser
"Replacing serine 742 within the IQ neck region with a phosphomimetic aspartic acid residue had no significant impact upon the affinity, calcium sensitivity or cooperative nature of the interaction between Myo1 and Cam1 ( xref )."
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sparser
"If TORC2 does indeed phosphorylate Myo1, it would be important to know, first, whether TORC2 function is elevated in meiotic cells compared to mitotic cells and, second, what the effect of different growth conditions (vegetative, sexual, and nutrient limiting) is on the association of Myo1 with Cam1 vs. Cam2 as assessed via co-immunoprecipitations."
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sparser
"Therefore, although Myo1 S742 phosphorylation does not impact the assembly of Myo1–Cam1 endocytic foci, it regulates myosin-1 to modulate the activity and function of the ensemble of endocytic proteins during bipolar growth."
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sparser
"Myo1 and Cam1 associated with the cell membrane in two distinct ways: we observed both (i) rapid, transient, binding of single Myo1 molecules to the cell membrane, characterized by low-intensity, single, stepwise, changes in intensity ( xref ), alongside (ii) longer endocytic events that were much brighter and took much longer ( xref )."
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