IndraLab

Statements

USP28 binds H2AC20. 5 / 5
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sparser
"To test if USP28 binds Histone H2A directly, the interaction between USP28 and histone H2A was further tested by GST pull-down assay."
30910399
sparser
"As shown in Fig. 4 C, both H2A and ub-K119-H2A were detected in the GST-USP28 pulldown complex, but not in the GST control complex, demonstrating that the interactions between USP28 and H2A is direct."
30910399
sparser
"Furthermore, interactions between USP28 and H2A was tested by the Ni-NTA pull down assay using his-tagged H2A and HA-tagged USP28."
30910399
sparser
"Similar to removing the K-48 linked ubiquitin chains of p53, studies have illustrated that USP28 can interact with H2A directly and reduce the level of ubiquitination at K119 of histone H2A (ub-K119-H2A) [ xref ]."
36879346
sparser
"To determine if USP28 directly interacts with H2A, immunoprecipitation assays were performed to detect interaction between the endogenous USP28 and H2A in U2OS cells."
30910399