IndraLab

Statements

USP21 is phosphorylated. 5 / 5
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rlimsp
"USP21 phosphorylation blocks its effect on Nanog."
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rlimsp
"(c) E14 cells were treated with mFGF4 (25 ng ml−1) in the presence of PD0325901 (1 μM) or not for 12 h. Phos-Tag SDS–PAGE was applied to detect the band shift of USP21 caused by phosphorylation."
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rlimsp
"Flag-USP21 was immunoprecipitated using the anti-Flag antibody and the phosphorylated USP21 was analysed with the anti-ERK1/2 phospho-substrate antibody."
27886188
rlimsp
"We analysed USP21 phosphorylation by using a phospho-Tag gel and found that both endogenous and exogenous USP21 were significantly phosphorylated upon the activation of ERK (Fig. 5c; Supplementary Fig. 5e)."
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rlimsp
"Moreover, differentiation cues promote ERK-mediated phosphorylation and dissociation of USP21 from Nanog, thus leading to Nanog degradation."
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